%A DHARMAWATI VERLI 
%T KEEFEKTIFAN EKSTRAK JAMUR ENDOFIT MANGROVE
SEBAGAI ANTIBAKTERI ALAMI TERHADAP
Vibrio alginolyticus DAN Photobacterium damselae spp.: STUDI DI
HUTAN MANGROVE PETENGORAN, PESAWARAN,

LAMPUNG
%X Agen penyakit bakterial seperti bakteri Vibrio alginolyticus dan Photobacterium
damselae spp. telah ditemukan menginfeksi ikan budi daya laut seperti kakap
putih bahkan sampai menimbulkan kematian. Eksplorasi alam guna mencari
sumber senyawa antimikroba baru terutama hewan akuatik masih terus dilakukan
terutama yang bersumber dari mikroba karena keragamannya yang tinggi namun
belum banyak diteliti. Mikroba banyak ditemukan berasosiasi dengan biota laut
dan tumbuhan di pesisir laut, seperti mangrove. Mangrove dikenal memiliki
kandungan senyawa bioaktif yang berkhasiat sebagai antibakteri begitu pula
dengan jamur endofit mangrove karena adanya transfer genetik antara jamur
endofit dan inang. Ekstrak jamur endofit dengan konsentrasi 500 ?g/disk dan
1000 ?g/disk yang diteteskan pada blank paper disc untuk pengujian aktivitas
antibakteri menggunakan metode disc diffusion. Hasil pengamatan diuji secara
statistik deskriptif dengan ANOVA pada level signifikansi p < 0,05 menunjukkan
bahwa aktivitas antibakteri terbaik terhadap V. alginolyticus dengan kategori
?sedang? didapatkan dari 4SLB1-1 (66,22?1,52%) pada konsentrasi 1000 ?g/disk
dan 4SLB1-4 (65,01?1,33 %) pada konsentrasi 1000 ?g/disk. Aktivitas antibakteri
terbaik terhadap P. damselae spp. dengan kategori ?kuat? didapatkan dari 4SLB1-
1 (99,18?0,53%) pada konsentrasi 1000 ?g/disk dan 4SLB1-4 (95,54?2,19 %)
pada konsentrasi 1000 ?g/disk. Identifikasi secara molekular menunjukkan bahwa
isolat 4 SLB1-1 memiliki kemiripan dengan jamur Pestalotiopsis rhizophorae dan
4SLB1-4 yang mirip dengan jamur Nigrospora magnoliae.
Kata kunci : antibakteri, endofit, mangrove, patogen, akuatik, Lampung


Bacterial disease agents such as bacteria Vibrio alginolyticus and Photobacterium
damselae spp. have been found to infect marine farmed fish such as sea bass and
even cause mortality. Exploration of nature to find sources of new antimicrobial
compounds, especially aquatic animals, is still being carried out, especially those
originating from microbes because of their high diversity but have yet to be
widely studied. Microbes are associated with marine biota and plants on the sea
coast, such as in mangroves. Previously, mangroves contained bioactive
compounds that have antibacterial properties as well as mangrove endophytic
fungi due to genetic transfer between endophytic fungi and the host. Endophytic
fungal extract with a concentration of 500 ?g/disk and 1000 ?g/disk was dripped
on a blank paper disc for testing antibacterial activity using the method of disc
diffusion. The observation results were tested descriptively statistically with
ANOVA at a significance level of p < 0.05. Best antibacterial activity against V.
alginolyticus in the "medium" category were 4SLB1-1 (66.22 ? 1.52%) at a
concentration of 1000 ?g/disc and 4SLB1-4 (65.01 ? 1.33 %) at a concentration of
1000 ?g/disk. Best antibacterial activity against P. damselae spp. in the "strong"
category were 4SLB1-1 (99.18?0.53%) at a concentration of 1000 ?g/disk and
4SLB1-4 (95.54?2.19%) at a concentration of 1000 ?g/disk. Molecular
identification showed that isolate 4 SLB1-1 had similarities to fungi Pestalotiopsis
rhizophorae and 4SLB1-4 had similarity with fungi Nigrospora magnoliae.

Keywords: antibacterial, endophytic, mangrove, pathogenic, aquatic, Lampung
%D 2024
%I UNIVERSITAS LAMPUNG
%L eprints84283