%0 Generic
%A Shinta , Nur’Aini.AS 
%C UNIVERSITAS LAMPUNG
%D 2024
%F eprints:84455
%I FAKULTAS PERTANIAN
%T STATUS KESEHATAN IKAN LELE Clarias gariepinus (BURCHELL,  1882) YANG DIBERI PAKAN BERBASIS TEPUNG DAGING DAN  TULANG DENGAN PENAMBAHAN DISTILLERS DRIED GRAINS WITH  SOLUBLES (DDGS), TAURIN, DAN PROBIOTIK Bacillus sp. SEBELUM  DAN SESUDAH INFEKSI Aeromonas hydrophila
%U http://digilib.unila.ac.id/84455/
%X Permintaan tepung ikan sebagai bahan baku pakan terus meningkat. Hal ini dibutuhkan  bahan baku pakan alternatif yaitu Distillers dried grain with solubles  (DDGS) serta MBM dengan penambahan taurin. Ikan lele sering terserang penyakit  Motile Aeromonas Septicemia (MAS). Penelitian ini bertujuan untuk menganalisis  pengaruh pemberian pakan berbasis tepung daging dan tulang dengan penambahan  DDGS, taurin, dan probiotik Bacillus sp. terhadap profil hematologi,  efisiensi pakan, dan retensi protein ikan lele. Penelitian ini menggunakan rancangan  acak kelompok (RAL) dengan 6 perlakuan dan masing-masing 3 ulangan:  P1(DDGS 0%+ taurin 0%), P2 (DDGS 5%+taurin 0,5 %), P3 (DDGS 5%+taurin  0%), P4 (DDGS 10% +taurin 0,5 %), P5 (DDGS 15%+taurin 1%), dan P6 (DDGS  20%+taurin 1,5%). Pemberian probiotik Bacillus sp. pada semua perlakuan sebanyak  10 ml/kg pakan. Panjang dan berat rata-rata ikan adalah 9,50±0,33 cm dan  5,77±0,69 g dipelihara selama 60 hari. Ikan sebanyak 5 ekor disampling pengambilan  darah sebelum dan setelah infeksi A. hydrophila dengan kepadatan bakteri  106 CFU/mL diambil pada H+1, H+3, dan H+5 setelah infeksi untuk dianalisis kesehatannya.  Variabel penelitian yang diamati: total eritrosit, total leukosit, kadar  hematokrit, aktivitas fagositosis, indeks fagositosis, efisiensi pakan (EP), retensi  protein (RP), dan tingkat kelangsungan hidup (SR). Hasil penelitian menunjukkan  bahwa perlakuan yang diberikan pada ikan memberikan pengaruh berbeda nyata  terhadap total eritrosit, total leukosit, dan aktivitas fagositosis dan tidak memberikan  pengaruh berbeda nyata terhadap EP, RP, kadar hematokrit, indeks fagositosis,  dan SR. Penelitian ini membuktikan bahwa pakan yang berbasis tepung daging  dan tulang, DDGS, taurin, dan probiotik Bacillus sp. dapat meningkatkan kesehatan  ikan lele (Clarias gariepinus).  Kata kunci: Aeromonas hydrophila, ikan lele, pakan, distillers dried grain with  solubles, Bacillus sp.    The demand for fish meal as a feed raw material continues to increase. This  requires alternative feed raw materials, namely Distillers dried grain with solubles  (DDGS) and MBM with the addition of taurine. Catfish are often attacked by  Motile Aeromonas Septicemia (MAS). This study aimed to analyze the effect of  providing meat and bone meal-based feed with the addition of DDGS, taurine, and  probiotic Bacillus sp. on the hematological profile, feed efficiency, and protein  retention of catfish. This study used a randomized block design (CRD) with 6  treatments and 3 replications each: P1 (DDGS 0% + taurine 0%), P2 (DDGS 5%  + taurine 0.5%), P3 (DDGS 5% + taurine 0%), P4 (DDGS 10% + taurine 0.5%),  P5 (DDGS 15% + taurine 1%), and P6 (DDGS 20% + taurine 1.5%). Probiotic  Bacillus sp. in all treatments as much as 10 ml/kg feed. The average length and  weight of the fish were 9.50±0.33 cm and 5.77±0.69 g maintained for 60 days.  Five fish were sampled for blood sampling before and after A. hydrophila  infection with a bacterial density of 106 CFU/mL taken on D+1, D+3, and D+5  after infection to analyze their health. The observed research variables: total  erythrocytes, total leukocytes, hematocrit levels, phagocytosis activity,  phagocytosis index, feed efficiency (EP), protein retention (RP), and survival rate  (SR). The results showed that the treatments given to the fish had a significantly  different effect on total erythrocytes, total leukocytes, and phagocytosis activity  and did not have a significantly different effect on EP, RP, hematocrit levels,  phagocytosis index, and SR. This study proves that feed based on meat and bone  meal, DDGS, taurine, and probiotic Bacillus sp. can improve the health of catfish  (Clarias gariepinus).  Keywords: Aeromonas hydrophila, catfish, feed, distillers dried grain with  solubles, Bacillus sp.