?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENINGKATAN+KESTABILAN+ENZIM+LIPASE+DARI+BAKTERI%0D%0AKlebsiella+sp.+LPG172+DENGAN+IMOBILISASI+METODE%0D%0AADSORPSI+PADA+MATRIKS+HIDROKSIAPATIT&rft.creator=Dyasmin+%2C+Dwi+Larasati&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Penelitian+ini+dilakukan+untuk+mempelajari+enzim+lipase+dari+bakteri%0D%0AKlebsiella+sp.+LPG172+terimobil+dengan+hidroksiapatit+mengenai+kemampuannya%0D%0Asebagai+katalis+pada+reaksi+hidrolisis+dan+transesterifikasi.+Pada+penelitian+ini%0D%0Adilakukan+peremajaan+dan+produksi+lipase+dari+bakteri+Klebsiella+sp.+LPG172%0D%0Ayang+memiliki+kondisi+optimum+pertumbuhan+pada+pH+7+dan+waktu+inkubasi+66%0D%0Ajam+pada+media+NA%2FNB+mengandung+minyak+zaitun%2C+serta+serangkaian+pemurnian%0D%0Aenzim+lipase+seperti+fraksinasi+ammonium+sulfat%2C+dialisis+dan+kromatografi+kolom%0D%0Afiltrasi+gel+sephadex+G-75+untuk+memperoleh+enzim+lipase+murni.+Kemudian%0D%0Adilakukan+imobilisasi+pada+matriks+hidroksiapatit%2C+ditentukan+kondisi+optimum%0D%0Aenzim+imobil+dan+dilakukan+pemakaian+berulang.%0D%0AEkstrak+kasar+enzim+memiliki+kadar+protein+sebesar+3%2C34+mg%2FmL+dan%0D%0Aaktivitas+unit+enzim+sebesar+34%2C6+U%2FmL.+Setelah+pemurnian+kromatografi+kolom%0D%0Afiltrasi+gel+sephadex+G-75+enzim+lipase+memiliki+kadar+protein+sebesar+0%2C70%0D%0Amg%2FmL+dan+aktivitas+unit+sebesar+1.401%2C67+U%2FmL+pada+uji+hidrolisis%2C+sedangkan%0D%0Auji+transesterifikasi+sebesar+1.723%2C6+U%2FmL.+Imobilisasi+lipase+pada+matriks%0D%0Ahidroksiapatit+menunjukkan+aktivitas+unit+sebesar+962%2C67+U%2FmL+pada+uji+hidrolisis%0D%0Adan+1.334%2C8+U%2FmL+pada+uji+transesterifikasi.%0D%0AImobilisasi+lipase+Klebsiella+sp.+LPG172+pada+matriks+hidroksiapatit%0D%0Amengubah+kondisi+optimum+aktivitas+enzim.+Pada+reaksi+hidrolisis+kondisi%0D%0Aoptimum+lipase+imobil+memiliki+pH+8%2C+suhu+50+%CC%8AC+dan+inkubasi+15+menit%0D%0Adibandingkan+enzim+bebasnya+yang+menunjukkan+aktivitas+optimum+pada+pH+7%2C%0D%0Asuhu+80+%CC%8AC%2C+dan+inkubasi+25+menit.+Pada+reaksi+transesterifikasi+memiliki+kondisi%0D%0Aoptimum+lipase+imobil+pH+8%2C+suhu+60%2C+dan+inkubasi+10+menit+dibandingkan+enzim%0D%0Abebasnya+yang+optimum+dengan+pH+7%2C+suhu+70+%CC%8AC%2C+dan+inkubasi+25+menit.+Kondisi%0D%0Aoptimum+enzim+imobil+tersebut+digunakan+untuk+pemakaian+berulang+yang+stabil%0D%0Apada+reaksi+hidrolisis+sebanyak+2+kali+pemakaian+dengan+aktivitas+sisa+sebesar%0D%0A30%25+dari+enzim+bebasnya+dan+aktivitas+enzim+imobil+sebesar+37%25+dari+enzim%0D%0Abebasnya+dipemakaian+ke+4+pada+reaksi+transesterifikasi.%0D%0A%0D%0AKata+kunci+%3A+Klebsiella+sp.+LPG172%2C+imobilisasi%2C+hidroksiapatit%2C+hidrolisis%2C%0D%0A%0D%0Atransesterifikasi.%0D%0A%0D%0A%0D%0AThis+study+was+conducted+to+study+lipase+from+Klebsiella+sp.+LPG172%0D%0Abacteria+immobilized+with+hydroxyapatite+regarding+its+ability+as+a+catalyst+in%0D%0Ahydrolysis+and+transesterification+reactions.+In+this+study%2C+the+rejuvenation+and%0D%0Aproduction+of+lipase+from+Klebsiella+sp.+LPG172+were+carried+out+which+had%0D%0Aoptimum+growth+conditions+at+pH+7+and+an+incubation+time+of+66+hours+in+NA%2FNB%0D%0Amedia+containing+olive+oil%2C+as+well+as+a+series+of+lipase+purifications+such+as%0D%0Aammonium+sulfate+fractionation%2C+dialysis+and+sephadex+G-75+gel+filtration+column%0D%0Achromatography+to+obtain+pure+lipase.+Then+immobilization+was+carried+out+on+the%0D%0Ahydroxyapatite+matrix%2C+the+optimum+conditions+of+the+immobilized+enzyme+were%0D%0Adetermined+and+repeated+use+was+carried+out.%0D%0AThe+crude+enzyme+extract+has+a+protein+content+of+3.34+mg%2FmL+and+an%0D%0Aenzyme+unit+activity+of+34.6+U%2FmL.+After+purification+by+sephadex+G-75+gel%0D%0Afiltration+column+chromatography%2C+the+lipase+has+a+protein+content+of+0.70+mg%2FmL%0D%0Aand+a+unit+activity+of+1%2C401.67+U%2FmL+in+the+hydrolysis+test%2C+while+the%0D%0Atransesterification+test+is+1%2C723.6+U%2FmL.+Immobilization+of+lipase+on%0D%0Ahydroxyapatite+matrix+shows+a+unit+activity+of+962.67+U%2FmL+in+the+hydrolysis+test%0D%0Aand+1%2C334.8+U%2FmL+in+the+transesterification+test.%0D%0AImmobilization+of+Klebsiella+sp.+LPG172+lipase+on+hydroxyapatite+matrix%0D%0Achanges+the+optimum+conditions+of+enzyme+activity.+In+the+hydrolysis+reaction%2C%0D%0Athe+optimum+conditions+of+immobilized+lipase+have+a+pH+8%2C+a+temperature+50+%CC%8AC%0D%0Aand+an+incubation+15+minutes+compared+to+the+free+enzyme+which+shows+optimum%0D%0Aactivity+at+pH+7%2C+a+temperature+80+%CC%8AC%2C+and+an+incubation+25+minutes.+In+the%0D%0Atransesterification+reaction%2C+the+optimum+conditions+of+immobilized+lipase+are+pH%0D%0A8%2C+a+temperature+60+%CC%8AC%2C+and+an+incubation+10+minutes+compared+to+the+free+enzyme%0D%0Awhich+is+optimum+at+pH+7%2C+a+temperature+70+%CC%8AC%2C+and+an+incubation+25+minutes.+The%0D%0Aoptimum+conditions+of+the+immobilized+enzyme+are+used+for+stable+repeated+use+in%0D%0Athe+hydrolysis+reaction+as+many+as+2+uses+with+a+residual+activity+of+30%25+of+the%0D%0Afree+enzyme+and+the+activity+of+the+immobilized+enzyme+is+37%25+of+the+free+enzyme%0D%0Ain+the+4th+use+in+the+transesterification+reaction.%0D%0AKeywords+%3A+Klebsiella+sp.+LPG172%2C+immobilization%2C+hydroxyapatite%2C+hydrolysis%2C%0D%0A%0D%0Atransesterification.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-08-21&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F84499%2F1%2FABSTRAK%2520-%2520Dyasmin%2520Dwi%2520Larasati.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F84499%2F2%2FSKRIPSI%2520FULL-1%2520-%2520Dyasmin%2520Dwi%2520Larasati.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F84499%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN-1%2520-%2520Dyasmin%2520Dwi%2520Larasati.pdf&rft.identifier=++Dyasmin+%2C+Dwi+Larasati++(2024)+PENINGKATAN+KESTABILAN+ENZIM+LIPASE+DARI+BAKTERI+Klebsiella+sp.+LPG172+DENGAN+IMOBILISASI+METODE+ADSORPSI+PADA+MATRIKS+HIDROKSIAPATIT.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F84499%2F