?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PEMURNIAN+ENZIM+KITINASE+SERTA+UJI+ANTIMIKROBA+DARI%0D%0A%0D%0AFUNGI+LAUT+18A12RF&rft.creator=Jordy+%2C+Setiawan&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Enzim+kitinase+merupakan+enzim+yang+spesifik+untuk+mendegradasi+kitin%0D%0Amenjadi+turunannya+seperti+chitooligosaccharide+(COS)+dan+monooligosaccharide%0D%0A(MOS).+Pada+penelitian+telah+dilakukan+peremajaan+dan+identifikasi+isolat+fungi%0D%0A18A12RF.+Penampakan+secara+visual%2C+fungi+memiliki+warna+putih+dengan+bentuk%0D%0Aseperti+kapas.+Sedangkan+secara+mikroskopi+isolat+18A12RF+terindikasi+sebagai%0D%0AAspergillus+sp.+Untuk+meperoleh+enzim+kitinase%2C+fungi+18A12RF+dikultivasi+secara%0D%0ASSF+pada+media+limbah+kulit+udang+selama+4+hari.%0D%0AOptimasi+produksi+enzim+kitinase+dilakukan+variasi+pH+5.+7%2C+dan+9.%0D%0ASelanjutnya%2C+hasil+kultivasi+diekstraksi+dengan+akuades+dan+dianlisis+kadar%0D%0Aglukosamin+menggunakan+DNS+dan+kadar+protein+menggunakan+Lowry.+Hasil%0D%0Aanalisis+menunjukan+bahwa+hasil+kultivasi+pada+pH+9+memiliki+kadar+tertinggi%0D%0Aglukosamin+(147%2C38+ppm).+Selanjutnya+proses+scale+up+dilakukan+menggunakan+100%0D%0Ag+kulit+udang+dalam+1000+mL+Erlenmeryer.+Hasil+Kultivasi+mengalami+peningkatan%0D%0Aaktivitas+sebanyak+6+kali+(0%2C5+U%2FmL).%0D%0AUji+antimikroba+enzim+kitinase+fungi+18A12RF+menunjukan+aktivitas%0D%0Aantijamur+terhadaap+Malassezia+globosa%2C+sedangkan+tidak+memiliki+aktivitas+terhadap%0D%0Abakteri+MDR+staphylococcus+aureus+dan+MDR+Pseudomonas+aeruginosa.+Enzim%0D%0Akitinase+yang+didapatkan+mampu+mendegradasi+substrat+menjadi+COS+dan+MOS%0D%0Adalam+waktu+inkubasi+2+jam%2C+didapatkan+nilai+Rf+yang+mendekati+standard+yaitu+0%2C4%3B%0D%0A0%2C3%3B+dan+0%2C4.+Hasil+degradasi+COS+juga+biasa+didapatkan+oleh+enzim+kitinase%2C%0D%0Asehingga+memudahkan+dalam+produksi+COS+sebagai+antibakteri.+Oleh+karena+itu%2C%0D%0Ainformasi+awal+ini+penting+untuk+kajian+lebih+lanjut+terkait+pemanfaatan+enzim%0D%0Akitinase+dari+isolat+fungi+perairan+Indonesia.%0D%0A%0D%0AKata+kunci+%3A+antimikroba%2C+enzim+kitinase%2C+isolat+fungi+laut+18A12RF%2C+solid+state%0D%0A%0D%0Afermentation%2Cglukosamin.%0D%0A%0D%0A%0D%0AChitinase+enzyme+is+a+specific+enzyme+for+degrading+chitin+into+its+derivatives%0D%0Asuch+as+chitooligosaccharide+(COS)+and+monooligosaccharide+(MOS).+In+this%0D%0Aresearch%2C+maintenance+and+identification+of+fungal+isolate+18A12RF+were+carried+out.%0D%0AVisually%2C+the+fungus+has+a+white+color+with+a+cotton-like+shape.+Meanwhile%2C%0D%0Amicroscopically%2C+isolate+18A12RF+was+indicated+as+Aspergillus+sp.+To+obtain+the%0D%0Achitinase+enzyme%2C+the+18A12RF+fungus+was+cultivated+using+SSF+in+shrimp+shell%0D%0Awaste+media+for+4+days.%0D%0AOptimization+of+chitinase+enzyme+production+was+carried+out+by+varying+pH%0D%0A5%2C+7%2C+and+9.+Then%2C+the+cultivation+results+were+extracted+with+aquadest+and+analyzed%0D%0Afor+glucosamine+content+using+DNS+and+protein+content+using+Lowry.+The+analysis%0D%0Aresults+showed+that+the+results+of+cultivation+at+pH+9+had+the+highest+levels+of%0D%0Aglucosamine+(147.38+ppm).+Furthermore%2C+the+scale+up+process+was+carried+out+using%0D%0A100+g+of+shrimp+shells+in+1000+mL+Erlenmeryer.+Cultivation+results+increased+activity%0D%0Aby+6+times+(0.5+U%2FmL).%0D%0AAntimicrobial+assay+of+the+fungal+chitinase+enzyme+18A12RF+showed%0D%0Aantifungal+activity+against+Malassezia+globosa%2C+while+it+had+no+activity+against+MDR%0D%0AStaphylococcus+aureus+and+MDR+Pseudomonas+aeruginosa+bacteria.+The+chitinase%0D%0Aenzyme+obtained+was+able+to+degrade+the+substrate+into+COS+and+MOS+within+an%0D%0Aincubation+time+of+2+hours.+The+Rf+value+was+obtained+which+was+close+to+the%0D%0Astandard%2C+namely+0.4%3B+0.3%3B+and+0.4.+COS+degradation+results+are+also+usually+obtained%0D%0Aby+the+chitinase+enzyme%2C+making+it+easier+to+produce+COS+as+an%0D%0Aantibacterial.Therefore%2C+this+initial+information+is+important+for+further+studies%0D%0Aregarding+the+utilization+of+chitinase+enzymes+from+Indonesian+waters%0D%0A%0D%0AKeyword+%3A+antimicrobial%2C+chitinase+enzyme%2C+marine+fungal+18A12RF%2C+solid+state%0D%0A%0D%0Afermentation%2Cglucosamine.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-08-07&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F84515%2F1%2FABSTRAK%2520-%2520Jordy%2520Setiawan.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F84515%2F2%2FSKRIPSI%2520FULL%2520-%2520Jordy%2520Setiawan.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F84515%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN%2520-%2520Jordy%2520Setiawan.pdf&rft.identifier=++Jordy+%2C+Setiawan++(2024)+PEMURNIAN+ENZIM+KITINASE+SERTA+UJI+ANTIMIKROBA+DARI+FUNGI+LAUT+18A12RF.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F84515%2F