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<http://digilib.unila.ac.id/id/eprint/84515> <http://purl.org/dc/terms/title> "PEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI\r\n\r\nFUNGI LAUT 18A12RF"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://purl.org/ontology/bibo/abstract> "Enzim kitinase merupakan enzim yang spesifik untuk mendegradasi kitin\r\nmenjadi turunannya seperti chitooligosaccharide (COS) dan monooligosaccharide\r\n(MOS). Pada penelitian telah dilakukan peremajaan dan identifikasi isolat fungi\r\n18A12RF. Penampakan secara visual, fungi memiliki warna putih dengan bentuk\r\nseperti kapas. Sedangkan secara mikroskopi isolat 18A12RF terindikasi sebagai\r\nAspergillus sp. Untuk meperoleh enzim kitinase, fungi 18A12RF dikultivasi secara\r\nSSF pada media limbah kulit udang selama 4 hari.\r\nOptimasi produksi enzim kitinase dilakukan variasi pH 5. 7, dan 9.\r\nSelanjutnya, hasil kultivasi diekstraksi dengan akuades dan dianlisis kadar\r\nglukosamin menggunakan DNS dan kadar protein menggunakan Lowry. Hasil\r\nanalisis menunjukan bahwa hasil kultivasi pada pH 9 memiliki kadar tertinggi\r\nglukosamin (147,38 ppm). Selanjutnya proses scale up dilakukan menggunakan 100\r\ng kulit udang dalam 1000 mL Erlenmeryer. Hasil Kultivasi mengalami peningkatan\r\naktivitas sebanyak 6 kali (0,5 U/mL).\r\nUji antimikroba enzim kitinase fungi 18A12RF menunjukan aktivitas\r\nantijamur terhadaap Malassezia globosa, sedangkan tidak memiliki aktivitas terhadap\r\nbakteri MDR staphylococcus aureus dan MDR Pseudomonas aeruginosa. Enzim\r\nkitinase yang didapatkan mampu mendegradasi substrat menjadi COS dan MOS\r\ndalam waktu inkubasi 2 jam, didapatkan nilai Rf yang mendekati standard yaitu 0,4;\r\n0,3; dan 0,4. Hasil degradasi COS juga biasa didapatkan oleh enzim kitinase,\r\nsehingga memudahkan dalam produksi COS sebagai antibakteri. Oleh karena itu,\r\ninformasi awal ini penting untuk kajian lebih lanjut terkait pemanfaatan enzim\r\nkitinase dari isolat fungi perairan Indonesia.\r\n\r\nKata kunci : antimikroba, enzim kitinase, isolat fungi laut 18A12RF, solid state\r\n\r\nfermentation,glukosamin.\r\n\r\n\r\nChitinase enzyme is a specific enzyme for degrading chitin into its derivatives\r\nsuch as chitooligosaccharide (COS) and monooligosaccharide (MOS). In this\r\nresearch, maintenance and identification of fungal isolate 18A12RF were carried out.\r\nVisually, the fungus has a white color with a cotton-like shape. Meanwhile,\r\nmicroscopically, isolate 18A12RF was indicated as Aspergillus sp. To obtain the\r\nchitinase enzyme, the 18A12RF fungus was cultivated using SSF in shrimp shell\r\nwaste media for 4 days.\r\nOptimization of chitinase enzyme production was carried out by varying pH\r\n5, 7, and 9. Then, the cultivation results were extracted with aquadest and analyzed\r\nfor glucosamine content using DNS and protein content using Lowry. The analysis\r\nresults showed that the results of cultivation at pH 9 had the highest levels of\r\nglucosamine (147.38 ppm). Furthermore, the scale up process was carried out using\r\n100 g of shrimp shells in 1000 mL Erlenmeryer. Cultivation results increased activity\r\nby 6 times (0.5 U/mL).\r\nAntimicrobial assay of the fungal chitinase enzyme 18A12RF showed\r\nantifungal activity against Malassezia globosa, while it had no activity against MDR\r\nStaphylococcus aureus and MDR Pseudomonas aeruginosa bacteria. The chitinase\r\nenzyme obtained was able to degrade the substrate into COS and MOS within an\r\nincubation time of 2 hours. The Rf value was obtained which was close to the\r\nstandard, namely 0.4; 0.3; and 0.4. COS degradation results are also usually obtained\r\nby the chitinase enzyme, making it easier to produce COS as an\r\nantibacterial.Therefore, this initial information is important for further studies\r\nregarding the utilization of chitinase enzymes from Indonesian waters\r\n\r\nKeyword : antimicrobial, chitinase enzyme, marine fungal 18A12RF, solid state\r\n\r\nfermentation,glucosamine."^^<http://www.w3.org/2001/XMLSchema#string> .
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<http://digilib.unila.ac.id/84515/11/small.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "small.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://eprints.org/ontology/hasDocument> <http://digilib.unila.ac.id/id/document/2332045> .
<http://digilib.unila.ac.id/id/document/2332045> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<http://digilib.unila.ac.id/id/document/2332045> <http://www.w3.org/2000/01/rdf-schema#label> "PEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI\r\n\r\nFUNGI LAUT 18A12RF (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/document/2332045> <http://eprints.org/relation/isVersionOf> <http://digilib.unila.ac.id/id/document/2331773> .
<http://digilib.unila.ac.id/id/document/2332045> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2331773> .
<http://digilib.unila.ac.id/id/document/2332045> <http://eprints.org/relation/isIndexCodesVersionOf> <http://digilib.unila.ac.id/id/document/2331773> .
<http://digilib.unila.ac.id/id/document/2332045> <http://eprints.org/ontology/hasFile> <http://digilib.unila.ac.id/84515/12/indexcodes.txt> .
<http://digilib.unila.ac.id/id/document/2332045> <http://purl.org/dc/terms/hasPart> <http://digilib.unila.ac.id/84515/12/indexcodes.txt> .
<http://digilib.unila.ac.id/84515/12/indexcodes.txt> <http://www.w3.org/2000/01/rdf-schema#label> "indexcodes.txt"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://eprints.org/ontology/hasDocument> <http://digilib.unila.ac.id/id/document/2332046> .
<http://digilib.unila.ac.id/id/document/2332046> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<http://digilib.unila.ac.id/id/document/2332046> <http://www.w3.org/2000/01/rdf-schema#label> "PEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI\r\n\r\nFUNGI LAUT 18A12RF (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/document/2332046> <http://eprints.org/relation/isVersionOf> <http://digilib.unila.ac.id/id/document/2331774> .
<http://digilib.unila.ac.id/id/document/2332046> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2331774> .
<http://digilib.unila.ac.id/id/document/2332046> <http://eprints.org/relation/isIndexCodesVersionOf> <http://digilib.unila.ac.id/id/document/2331774> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://eprints.org/ontology/hasDocument> <http://digilib.unila.ac.id/id/document/2332047> .
<http://digilib.unila.ac.id/id/document/2332047> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<http://digilib.unila.ac.id/id/document/2332047> <http://www.w3.org/2000/01/rdf-schema#label> "PEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI\r\n\r\nFUNGI LAUT 18A12RF (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/document/2332047> <http://eprints.org/relation/isVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332047> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332047> <http://eprints.org/relation/isIndexCodesVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332047> <http://eprints.org/ontology/hasFile> <http://digilib.unila.ac.id/84515/14/indexcodes.txt> .
<http://digilib.unila.ac.id/id/document/2332047> <http://purl.org/dc/terms/hasPart> <http://digilib.unila.ac.id/84515/14/indexcodes.txt> .
<http://digilib.unila.ac.id/84515/14/indexcodes.txt> <http://www.w3.org/2000/01/rdf-schema#label> "indexcodes.txt"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://eprints.org/ontology/hasDocument> <http://digilib.unila.ac.id/id/document/2332048> .
<http://digilib.unila.ac.id/id/document/2332048> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<http://digilib.unila.ac.id/id/document/2332048> <http://www.w3.org/2000/01/rdf-schema#label> "PEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI\r\n\r\nFUNGI LAUT 18A12RF (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/document/2332048> <http://eprints.org/relation/isVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332048> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332048> <http://eprints.org/relation/islightboxThumbnailVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332048> <http://eprints.org/ontology/hasFile> <http://digilib.unila.ac.id/84515/15/lightbox.jpg> .
<http://digilib.unila.ac.id/id/document/2332048> <http://purl.org/dc/terms/hasPart> <http://digilib.unila.ac.id/84515/15/lightbox.jpg> .
<http://digilib.unila.ac.id/84515/15/lightbox.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "lightbox.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://eprints.org/ontology/hasDocument> <http://digilib.unila.ac.id/id/document/2332049> .
<http://digilib.unila.ac.id/id/document/2332049> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<http://digilib.unila.ac.id/id/document/2332049> <http://www.w3.org/2000/01/rdf-schema#label> "PEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI\r\n\r\nFUNGI LAUT 18A12RF (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/document/2332049> <http://eprints.org/relation/isVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332049> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332049> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332049> <http://eprints.org/ontology/hasFile> <http://digilib.unila.ac.id/84515/16/preview.jpg> .
<http://digilib.unila.ac.id/id/document/2332049> <http://purl.org/dc/terms/hasPart> <http://digilib.unila.ac.id/84515/16/preview.jpg> .
<http://digilib.unila.ac.id/84515/16/preview.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "preview.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://eprints.org/ontology/hasDocument> <http://digilib.unila.ac.id/id/document/2332050> .
<http://digilib.unila.ac.id/id/document/2332050> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<http://digilib.unila.ac.id/id/document/2332050> <http://www.w3.org/2000/01/rdf-schema#label> "PEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI\r\n\r\nFUNGI LAUT 18A12RF (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/document/2332050> <http://eprints.org/relation/isVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332050> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332050> <http://eprints.org/relation/ismediumThumbnailVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
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<http://digilib.unila.ac.id/id/document/2332050> <http://purl.org/dc/terms/hasPart> <http://digilib.unila.ac.id/84515/17/medium.jpg> .
<http://digilib.unila.ac.id/84515/17/medium.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "medium.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://eprints.org/ontology/hasDocument> <http://digilib.unila.ac.id/id/document/2332051> .
<http://digilib.unila.ac.id/id/document/2332051> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<http://digilib.unila.ac.id/id/document/2332051> <http://www.w3.org/2000/01/rdf-schema#label> "PEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI\r\n\r\nFUNGI LAUT 18A12RF (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/document/2332051> <http://eprints.org/relation/isVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332051> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332051> <http://eprints.org/relation/issmallThumbnailVersionOf> <http://digilib.unila.ac.id/id/document/2331775> .
<http://digilib.unila.ac.id/id/document/2332051> <http://eprints.org/ontology/hasFile> <http://digilib.unila.ac.id/84515/18/small.jpg> .
<http://digilib.unila.ac.id/id/document/2332051> <http://purl.org/dc/terms/hasPart> <http://digilib.unila.ac.id/84515/18/small.jpg> .
<http://digilib.unila.ac.id/84515/18/small.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "small.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/84515> <http://www.w3.org/2000/01/rdf-schema#seeAlso> <http://digilib.unila.ac.id/84515/> .
<http://digilib.unila.ac.id/84515/> <http://purl.org/dc/elements/1.1/title> "HTML Summary of #84515 \n\nPEMURNIAN ENZIM KITINASE SERTA UJI ANTIMIKROBA DARI&#13;\n&#13;\nFUNGI LAUT 18A12RF\n\n" .
<http://digilib.unila.ac.id/84515/> <http://purl.org/dc/elements/1.1/format> "text/html" .
<http://digilib.unila.ac.id/84515/> <http://xmlns.com/foaf/0.1/primaryTopic> <http://digilib.unila.ac.id/id/eprint/84515> .
<http://digilib.unila.ac.id/id/subject/500> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.w3.org/2004/02/skos/core#Concept> .
<http://digilib.unila.ac.id/id/subject/500> <http://www.w3.org/2004/02/skos/core#prefLabel> "500 ilmu pengetahuan alam dan matematika" .
<http://digilib.unila.ac.id/id/eprint/84515> <http://purl.org/dc/terms/subject> <http://digilib.unila.ac.id/id/subject/500> .
<http://digilib.unila.ac.id/id/subject/540> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://www.w3.org/2004/02/skos/core#Concept> .
<http://digilib.unila.ac.id/id/subject/540> <http://www.w3.org/2004/02/skos/core#prefLabel> "540 Kimia dan ilmu yang berhubungan" .
<http://digilib.unila.ac.id/id/eprint/84515> <http://purl.org/dc/terms/subject> <http://digilib.unila.ac.id/id/subject/540> .