@misc{eprints84612,
           month = {Desember},
           title = {RESPON IMUN NON-SPESIFIK IKAN LELE (Clarias sp.) YANG DIBERI PAKAN BERBAHAN BAKU Distillers Dried Grains with Solubles (DDGS) DAN TAURIN},
          author = {AMELIA  RINDI },
         address = {UNIVERSITAS LAMPUNG},
       publisher = {FAKULTAS PERTANIAN},
            year = {2024},
             url = {http://digilib.unila.ac.id/84612/},
        abstract = {Distillers Dried Grains with Solubles (DDGS) dan taurin merupakan bahan pakan
potensial sebagai bahan pakan alternatif pengganti tepung ikan guna mencegah serangan
penyakit. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian
pakan berbahan baku DDGS dan taurin terhadap respon imun non-spesifik ikan
lele sebelum dan sesudah infeksi bakteri Staphylococcus aureus. Metode penelitian
menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan (DDGS\% :
taurin\%): Kontrol: (0 : 0); P1: (5 : 0,5); P2: (5 : 0); P3: (10 : 0,5); P4: (15 : 1,0);
P5: (20 : 1,5) dan 3 ulangan yang diberikan pada ikan lele dengan berat dan panjang
awal 4,06 g dan 9,20 cm selama 60 hari. Lalu, dilanjutkan dengan uji tantang
selama 7 hari. Sampling respon imun non-spesifik dilakukan pada H60 dan pasca
uji tantang pada H1, H3, dan H5 dengan diambil 6 dan 3 ekor pada masingmasing
perlakuan. Hasil penelitian menunjukkan bahwa perlakuan yang diberikan
setelah 60 hari memberikan pengaruh berbeda nyata terhadap kadar hematokrit
(14,35-22,34\%), total eritrosit (2,47-9,70 {$\times$}105sel/mm3), aktivitas fagositosis
(30,00-47,67\%) dan indeks fagositosis (1,41-1,71) (P{\ensuremath{<}}0.05) dan tidak memberikan
pengaruh berbeda nyata terhadap total leukosit (43,63-55,48 {$\times$}103sel/mm3)
(P{\ensuremath{>}}0.05) ikan lele. Kemudian, pasca uji tantang kelima variabel (kadar hematokrit:
14,52-23,31\%; total eritrosit: 3,40-9,77 {$\times$}105sel/mm3; total leukosit: 32,68-
59,37 {$\times$}103sel/mm3; aktivitas fagositosis: 18,00-44,33\%; indeks fagositosis: 1,29-
1,77) tidak dipengaruhi oleh perlakuan. Kesimpulan mengindikasikan bahwa perlakuan
yang diujikan dapat meningkatkan respon imun non-spesifik ikan lele dan
setelah diinfeksi bakteri Staphylococcus aureus menunjukkan kemampuan memproduksi
imun non-spesifik yang sama untuk semua perlakuan.
Kata kunci : DDGS, Pakan ikan, respon imun non-spesifik, Staphylococcus
aureus, taurin.

Distillers Dried Grains with Solubles (DDGS) and taurine are potential feed
ingredients as alternative feed ingredients as replace fish meal to prevent fish
disease attacks. The study aimed to evaluate the effect of feeding DDGS and
taurine on the non-specific immune response toward Staphylococcus aureus
bacterial infection of catfish juvenile. The research method used a completely
randomized design (CRD) with 6 treatments (DDGS\%: taurine\%): Control: (0: 0);
P1: (5: 0.5); P2: (5: 0); P3: (10: 0.5); P4: (15: 1.0); P5: (20: 1.5) and 3 replications
given to catfish with an initial weight and length of 4.06 g and 9.20 cm for 60
days. Then, continued with a challenge test for 7 days. Sampling of non-specific
immune responses was conducted on H60 and post-challenge test on H1, H3, and
H5 by taking 6 and 3 fish in each treatment. The results showed that the treatment
had a significantly different effect on hematocrit levels (14.35-22.34\%), total
erythrocytes (2.47-9.70 {$\times$} 105 cells/mm3), phagocytosis activity (30.00-47.67\%)
and phagocytosis index (1.41-1.71) (P{\ensuremath{<}}0.05) and did not have a significantly
different effect on total leukocytes (43.63-55.48 {$\times$} 103 cells/mm3) (P{\ensuremath{>}}0.05) of
catfish. Then, after the challenge test, the five variables (hematocrit level: 14.52-
23.31\%; total erythrocytes: 3.40-9.77 {$\times$}105 cells/mm3; total leukocytes: 32.68-
59,37 {$\times$}103 cells/mm3; phagocytosis activity: 18.00-44.33\%; phagocytosis index:
1.29-1.77) were not affected by the treatment. The conclusion indicates that the
treatment can increase the non-specific immune response of catfish juvenile and
after being infected with Staphylococcus aureus bacteria showed the same ability
to produce immunity response.
Keywords: DDGS, Fish feed, non-specific immune response, Staphylococcus
aureus, taurine.}
}