%0 Generic
%A Sri 	, Ajeng Kholifatun Nisa
%C UNIVERSITAS LAMPUNG
%D 2024
%F eprints:84798
%I FAKULTAS PERTANIAN
%T UJI HOMOGENITAS DAN UJI STABILITAS PATOGEN IKAN  Aeromonas hydrophila (Stanier, 1943) DALAM MEDIA TRANSPORT    AMIES
%U http://digilib.unila.ac.id/84798/
%X Kegiatan budi daya ikan air tawar terancam oleh infeksi penyakit yang disebabkan  oleh Aeromonas hydrophila. Isolat bakteri ini sering digunakan sebagai bahan    praktikum, penelitian, kontrol kualitas di laboratorium, dan plasma nutfah. Peneli-  tian mengenai pengaruh durasi transportasi isolat bakteri, penentuan suhu dan    waktu inkubasi isolat Aeromonas hydrophila dengan media transport amies untuk    menguji stabilitas dan homogenitasnya masih sangat terbatas. Penelitian ini dila-  kukan untuk mengevaluasi homogenitas dan stabilitas isolat Aeromonas hydrophi-  la yang dipreservasi di media transport amies setelah ditransportasikan dengan    perbedaan durasi transportasi, suhu, dan waktu inkubasi. Isolat yang digunakan    adalah ATCC Aeromonas hydrophila 7966 dan isolat lokal I/685. Penelitian dila-  kukan di Balai Pengujian Kesehatan Ikan dan Lingkungan (BPKIL) Serang. Para-  meter pada penelitian ini adalah uji homogenitas dan uji stabilitas dengan mem-  perhatikan viabilitas serta deteksi Aeromonas hydrophila. Hasil yang didapatkan    adalah 56 bahan uji dari dua isolat utama 100% homogen, 100% stabil setelah di-  transportasikan ke BBPBAT Sukabumi Jawa Barat, BPBAT Mandiangin Kali-  mantan Selatan, BPBAP Aceh, serta disimpan pada suhu 4oC, 25oC, dan 37oC se-  lama empat minggu.    Kata kunci: Aeromonas hydrophila, media transport amies, homogenitas, stabi-  litas  Fish farming activities in fresh water was threat by diseases caused by Aeromonas    hydrophila. This bacterial isolate is often used as material for practical work, re-  search, quality control in laboratory, and germplasm. Research on the duration of    transportation of bacterial isolates, optimal temperature and incubation period for  Aeromonas hydrophila isolates with amies transport medium to test its stability    and homogeneity are still limited. This research was conducted to evaluated ho-  mogeneity and stability of Aeromonas hydrophila isolates preserved in the amies    transport medium after transportation with differences in transportation duration,    temperature, and incubation time. The isolates used were ATCC Aeromonas hyd-  rophila 7966 and local isolate I/685. The research was conducted at Fish and En-  vironmental Health Testing Center (BPKIL) Serang. The parameters in this study    were homogeneity and stability tests taking into account the viability and detec-  tion of Aeromonas hydrophila. The results obtained were that 56 test materials    from the two main isolates were 100% homogeneous, 100% stable after trans-  ported to BBPBAT Sukabumi West Java, BPBAT Mandiangin South Kalimantan,    BPBAP Aceh, and stored at 4oC, 25oC and 37oC for four weeks.  Keyword: Aeromonas hydrophila, amies transport medium, homogeneity, stability