?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=UJI+KUALITATIF+KOPI+ROBUSTA+(Coffea+canephora)+DENGAN%0D%0APENANDA+GEN+N-METHYLTRANSFERASE+di+KTH+BUMI+MULYO%2C%0D%0AGAPOKTANHUT+LESTARI+SEJAHTERA%2C+KPHL+KOTAAGUNG+UTARA%2C%0D%0A%0D%0ATANGGAMUS&rft.creator=Andriyani+Wijaya%2C+Kusuma&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=570+Biologi&rft.description=Kopi+menjadi+salah+satu+komoditas+perkebunan+yang+berkembang+dengan+nilai%0D%0Aekonomi+cukup+tinggi+dan+tersebar+luas+di+berbagai+negara+termasuk+Indonesia.%0D%0ALampung+merupakan+sentra+produksi+kopi+terbesar+kedua+setelah+Sumatera+Selatan.%0D%0AMayoritas+petani+kopi+di+Lampung+menanam+kopi+robusta+dibandingkan+dengan%0D%0Akopi+arabika.+Masyarakat+yang+berperan+sebagai+petani+penggarap+budidaya+kopi%0D%0Arobusta+di+KTH+Bumi+Mulyo%2C+Gapoktanhut+Lestari+Sejahtera+memiliki+izin%0D%0Amengelola+hutan+kemasyarakatan+di+Kawasan+Register+31+Pematang+Arahan+dengan%0D%0Akewajiban+tata+kelola+kawasan+untuk+melindungi+lingkungan+dan+keanekaragaman%0D%0Ahayati.+Informasi+keragaman+genetik+tanaman+kopi+pada+tingkat+individu%2C+spesies%2C%0D%0Amaupun+populasi+perlu+diketahui+sebagai+dasar+pertimbangan+dalam+menyusun%0D%0Astrategi+konservasi%2C+pemuliaan%2C+pengelolaan+serta+pemanfaatan+sumberdaya+genetik%0D%0Atanaman+secara+berkelanjutan.+Upaya+konservasi+keragaman+genetik+kopi+saat+ini%0D%0Aperlu+dilakukan+dengan+penandaan+berbasis+molekuler+untuk+konfirmasi+spesies%0D%0Akopi+robusta+dan+sebagai+data+dasar+plasma+nutfah+Indonesia.+Penelitian+ini%0D%0Abertujuan+untuk+menguji+keberhasilan+amplifikasi+gen+N-Methyltransferase+sebagai%0D%0Alangkah+awal+analisis+molekuler+untuk+konfirmasi+spesies+kopi+robusta+dalam%0D%0Apenyusunan+data+keanekaragaman+hayati+di+KTH+Bumi+Mulyo%2C+Gapoktanhut%0D%0ALestari+Sejahtera%2C+KPHL+Kota+Agung+Utara%2C+Tanggamus.+Penelitian+dilakukan%0D%0Amelalui+beberapa+tahapan%2C+yaitu+isolasi+DNA%2C+amplifikasi+DNA%2C+elektroforesis+dan%0D%0Avisualisasi.+Tahapan+penelitian+dilaksanakan+di+Laboratorium+Bioteknologi+Balai%0D%0AVeteriner+Lampung.+Hasil+penelitian+menyatakan+bahwa+uji+kualitas+amplifikasi%0D%0Agen+N-Methyltransferase+berhasil+dilakukan+pada+suhu+annealing+optimal+60oC%0D%0Adengan+adanya+pendaran+pita+DNA+yang+terletak+pada+ukuran+1858+bp%2C+ditunjukkan%0D%0Apada+hasil+visualisasi+UV-Vis+dalam+elektroforesis.%0D%0AKata+kunci%3A+analisis+molekuler%2C+daun+kopi+robusta%2C+Gapoktanhut+Lestari+Sejahtera%2C%0D%0A%0D%0Akonservasi%2C+KTH+Bumi+Mulyo.%0D%0A%0D%0ACoffee+has+become+one+of+the+plantation+commodities+that+has+developed+with+a%0D%0Afairly+high+economic+value+and+is+widely+spread+in+various+countries%2C+including%0D%0AIndonesia+Lampung+is+the+second+largest+coffee+production+center+after+South%0D%0ASumatra.+The+majority+of+coffee+farmers+in+Lampung+cultivate+robusta+coffee%0D%0Acompared+to+arabica+coffee.+The+community+members+who+play+the+role+of+coffee%0D%0Afarmers+in+KTH+Bumi+Mulyo%2C+Gapoktanhut+Lestari+Sejahtera%2C+have+permission+to%0D%0Amanage+the+community+forest+in+Register+31+Pematang+Arahan+area%2C+with+the%0D%0Aobligation+to+govern+the+area+to+protect+the+environment+and+biodiversity.%0D%0AKnowledge+of+genetic+diversity+in+coffee+plants+at+the+individual%2C+species%2C+and%0D%0Apopulation+levels+is+essential+as+a+foundation+for+considering+conservation%2C%0D%0Abreeding%2C+management%2C+and+sustainable+utilization+of+plant+genetic+resources.%0D%0AConservation+efforts+for+coffee+genetic+diversity+currently+need+to+be+carried+out%0D%0Athrough+molecular-based+labeling+to+confirm+robusta+coffee+species+and+as%0D%0Afundamental+data+for+Indonesian+germplasm.+This+research+aims+to+test+the+success%0D%0Aof+N-Methyltransferase+gene+amplification+as+an+initial+step+in+molecular+analysis%0D%0Afor+confirming+robusta+coffee+species+in+compiling+biodiversity+data+at+KTH+Bumi%0D%0AMulyo%2C+Gapoktanhut+Lestari+Sejahtera%2C+KPHL+Kota+Agung+Utara%2C+Tanggamus.+The%0D%0Aresearch+was+conducted+through+several+stages%2C+namely+DNA+isolation%2C+DNA%0D%0Aamplification%2C+electrophoresis%2C+and+visualization.+The+research+stages+are+conducted%0D%0Aat+the+Biotechnology+Laboratory+of+the+Lampung+Veterinary+Center.+The+research%0D%0Afindings+indicate+that+the+quality+test+of+N-Methyltransferase+gene+amplification%0D%0Awas+successfully+conducted+at+the+optimal+annealing+temperature+of+60%C2%B0C%2C+with+the%0D%0Apresence+of+a+DNA+band+observed+at+a+size+of+1858+bp%2C+as+shown+in+the+UV-Vis%0D%0Avisualization+results+during+electrophoresis.%0D%0AKeywords%3A+conservation%2C+Gapoktanhut+Lestari+Sejahtera%2C+KTH+Bumi+Mulyo%2C%0D%0A%0D%0Amolecular+analysis%2C+robusta+coffee+leaves&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-07-18&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F84931%2F1%2FABSTRAK%2520-%2520Andriyani%2520Wijaya.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F84931%2F2%2FSKRIPSI%2520FULL%2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