?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=ISOLASI+SEKUENS+GEN+POLYKETIDE+SYNTHASE+(PKS)+DARI%0D%0AAKTINOBAKTERI+Pseudonocardia+carboxydivorans+18A13O1+YANG%0D%0A%0D%0ABERSIMBIOSIS+DENGAN+SPONS&rft.creator=DIANA%2C+SALSABILA&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=570+Biologi&rft.description=Drug-resistant+bacteria+pose+a+serious+risk+to+people's+health%2C+making+it+necessary+to%0D%0Asearch+for+new+antibacterials+from+natural+sources+such+as+marine+sponges.+The%0D%0Abioactive+compounds+of+sponges+are+mostly+produced+by+microbial+symbionts%2C%0D%0Aespecially+actinobacteria%2C+which+are+well-known+for+their+high+G%2BC+content+and%0D%0Avariety+of+bioactive+compounds.+Pseudonocardia+carboxydivoran+is+one+species+of%0D%0Amarine+actinobacteria+that+has+the+potential+to+produce+bioactive+compounds.+These%0D%0Acompounds+are+often+complex+polyketides+synthesised+by+the+polyketide+synthase%0D%0A(PKS)+gene.+Therefore%2C+screening+of+PKS+genes+can+reveal+the+potential+for+new%0D%0Abioactive+compounds.+This+study+aimed+to+isolate+the+polyketide+synthase+(PKS)%0D%0Agene+sequence+from+the+marine+actinobacteria+Pseudonocardia+carboxydivorans%0D%0A18A13O1+associated+with+sponges.+Samples+were+enriched+on+1%25+colloidal+chitin%0D%0Aagar+medium%2C+followed+by+microscopic+observation+to+ensure+the+purity+of+the%0D%0Aisolates.+Afterwards%2C+the+isolates+were+subcultured+on+liquid+ISP-2+medium+and%0D%0Aincubated+for+7+days.+DNA+was+extracted+using+PROMEGA+Kit+and+analysed+for%0D%0Aconcentration+and+purity+with+a+nanophotometer.+Subsequently%2C+PKS+sequences%0D%0Awere+amplified+using+primers+PKS1.+Amplification+results+were+visualised+by+1.2%25%0D%0Aagarose+gel+electrophoresis.+Sequencing+was+performed+by+the+Sanger+method.%0D%0ASequencing+results+were+analysed+using+BioEdit+and+Open+Reading+Frame+(ORF)-%0D%0AFinder+from+NCBI.+The+results+showed+that+the+PKS+gene+sequences+obtained%0D%0Aencoded+hexokinase+proteins%2C+which+are+known+to+contribute+to+the+production+of%0D%0Aantibiotic+compounds.%0D%0A%0D%0AKeywords%3A+Pseudonocardia+carboxydivorans%2C+antibacterial%2C+PKS%2C+PCR%2C%0D%0A%0D%0AHexokinase%0D%0A%0D%0ABakteri+resisten+obat+menimbulkan+risiko+serius+bagi+kesehatan+masyarakat%2C%0D%0Asehingga+diperlukan+pencarian+antibakteri+baru+dari+sumber+alami+seperti+spons+laut.%0D%0ASenyawa+bioaktif+spons+sebagian+besar+dihasilkan+oleh+mikroba+simbion%2C%0D%0Akhususnya+aktinobakteri%2C+yang+terkenal+dengan+kandungan+G%2BC+tinggi+dan+variasi%0D%0Asenyawa+bioaktifnya.+Pseudonocardia+carboxydivoran+merupakan+salah+satu+jenis%0D%0Aaktinobakteri+laut+yang+berpotensi+menghasilnya+senyawa+bioaktif.+Senyawa+ini%0D%0Asering+merupakan+poliketida+kompleks+yang+disintesis+oleh+gen+polyketide+synthase%0D%0A(PKS).+Oleh+karena+itu%2C+skrining+gen+PKS+dapat+mengungkap+potensi+senyawa%0D%0Abioaktif+baru.+Penelitian+ini+bertujuan+mengisolasi+sekuen+gen+polyketide+synthase%0D%0A(PKS)+dari+aktinobakteri+laut+Pseudonocardia+carboxydivorans+18A13O1+yang%0D%0Abersimbiosis+dengan+spons.+Sampel+diremajakan+pada+media+1%25+koloid+kitin+agar%2C%0D%0Adilanjutkan+dengan+pengamatan+mikroskopis+untuk+memastikan+kemurnian+isolat.%0D%0AKemudian%2C+isolat+disubkultur+pada+media+ISP-2+cair+dan+diinkubasi+selama+7+hari.%0D%0ADNA+diekstraksi+menggunakan+Kit+PROMEGA+dan+dianalisis+konsentrasi+dan%0D%0Akemurniannya+dengan+nanofotometer.+Kemudian+sekuen+PKS+diamplifikasi%0D%0Amenggunakan+primer+PKS1.+Hasil+amplifikasi+divisualisasi+dengan+elektroforesis%0D%0Agel+Agarosa+1%2C2%25.+Sekuensing+dilakukan+dengan+metode+Sanger.+Hasil+sekunsing%0D%0Adianalisis+menggunakan+BioEdit+dan+laman+bioinformatika+Open+Reading+Frame%0D%0A(ORF)-Finder+NCBI.+Hasil+penelitian+menunjukkan+bahwa+sekuens+gen+PKS+yang%0D%0Adiperoleh+mengkodekan+protein+heksokinase%2C+yang+diketahui+berkontribusi+dalam%0D%0Aproduksi+senyawa+antibiotik.%0D%0AKata+kunci%3A+Pseudonocardia+carboxydivorans%2C+antibakteri%2C+PKS%2C+PCR%2C%0D%0A%0D%0AHeksokinase&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-07-17&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F85200%2F1%2FABSTRAK%2520-%2520DIANA%2520SALSABILA%2520-%2520Diana%2520Salsabila.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F85200%2F2%2FFULL%2520SKRIPSI%2520TANPA%2520LAMPIRAN%2520-%25202017020173%2520-%2520DIANA%2520SALSABILA%2520-%2520Diana%2520Salsabila.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F85200%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN%2520-%25202017021073%2520-%2520DIANA%2520SALSABILA%2520-%2520Diana%2520Salsabila.pdf&rft.identifier=++DIANA%2C+SALSABILA++(2024)+ISOLASI+SEKUENS+GEN+POLYKETIDE+SYNTHASE+(PKS)+DARI+AKTINOBAKTERI+Pseudonocardia+carboxydivorans+18A13O1+YANG+BERSIMBIOSIS+DENGAN+SPONS.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F85200%2F