@misc{eprints86277,
           month = {Maret},
           title = {AKTIVITAS KOAGULASI HEMOLIM LOBSTER PASIR (Panulirus
homarus) DENGAN PENAMBAHAN FUKOIDAN DAN UJI AGLUTINASI
SERTA EKSPRESI GEN UNTUK DETEKSI ENDOTOKSIN PENYAKIT
BAKTERI PATOGEN PADA IKAN},
          author = {SIMAMORA PETRA TAMARA },
         address = {UNIVERSITAS LAMPUNG},
       publisher = {FAKULTAS PERTANIAN},
            year = {2025},
             url = {http://digilib.unila.ac.id/86277/},
        abstract = {Lobster hemolymph is explored as an endotoxin detector and an
alternative antibacterial test due to its sensitivity to lipopolysaccharides (LPS),
similar to horseshoe crabs. Lobster hemolymph clots quickly, requiring an
alternative anticoagulant like fucoidan. This study aimed to evaluate the
anticoagulant activity of fucoidan from brown algae using the Lee-White
coagulation test, agglutination test, and gene expression analysis in lobster
hemolymph for detecting endotoxins from bacteria that cause diseases in fish.
This study employs an exploratory method by screening different types and doses
of anticoagulants using six treatments: control, addition of 10\% aspirin, 10\%
EDTA, 10\% sodium citrate, heparin, and fucoidan to lobster hemolymph. The
coagulation time of the lobster hemolymph was measured, and an agglutination
test was observed after the addition of fish pathogenic bacteria. The results of the
Lee-White test showed that the hemolymph-to-fucoidan ratios of 2:3, 2:4, and 2:5
influenced coagulation. The agglutination test results indicated agglutination
formation in all samples. The cycle threshold (CT) values for gene expression
analysis were as follows: {\ensuremath{\beta}}-Actin 1 at 34.9226, {\ensuremath{\beta}}-Actin 2 at 29.8296, GPO at
42.042, Lectin 1 at 41.937, Lectin 2 at 42.1846, LGBP 1 at 39.9524, and LGBP 2
at 35.8335. In conclusion, fucoidan can be used as an anticoagulant at a 2:3 ratio,
and lobster hemolymph has the potential to be utilized as an antibacterial agent
against fish pathogens. Additionally, the genes {\ensuremath{\beta}}-Actin, LGBP, Lectin, and GPO
were successfully expressed in the hemolymph of the sand lobster (Panulirus
homarus).
Keywords: Agglutination Test, Coagulation Test, Endotoxins, Fucoidan, Gene
Expression, Spiny Lobster

Hemolim lobster dieksplorasi sebagai detektor endotoksin dan alternatif
uji antibakteri karena sensitif terhadap lipopolisakarida (LPS), mirip dengan
kepiting tapal kuda. Hemolim lobster cepat menggumpal, sehingga dibutuhkan
alternatif antikoagulan seperti fukoidan. Tujuan dari penelitian ini adalah untuk
menguji aktivitas antikoagulan senyawa fukoidan dari alga coklat menggunakan
uji koagulasi, uji aglutinasi serta ekspresi gen pada hemolim lobster sebagai
deteksi endotoksin bakteri penyebab penyakit ikan. Penelitian ini menggunakan
metode eksploratif yaitu dengan skrining jenis dan dosis antikoagulan
menggunakan 6 perlakuan yaitu kontrol, penambahan aspirin 10\%, EDTA 10\%,
natrium sitrat 10\%, heparin dan fukoidan ke hemolim lobster pasir (Panulirus
homarus) lalu dilakukan perhitungan lama hemolim lobster menggumpal dan
diamati uji agutinasi yang ditambah bakteri penyebab penyakit ikan. Hasil
penelitian uji koagulasi menunjukkan bahwa perbandingan hemolim dengan
fukoidan adalah 2:3, 2:4 dan 2:5, untuk hasil uji aglutinasi menunjukkan
semuanya mengalami pembentukan aglutinasi, dan nilai CT ekspresi gen sampel
}
}