?url_ver=Z39.88-2004&rft_id=2054111004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=AKTIVITAS+KOAGULASI+HEMOLIM+LOBSTER+PASIR+(Panulirus%0D%0Ahomarus)+DENGAN+PENAMBAHAN+FUKOIDAN+DAN+UJI+AGLUTINASI%0D%0ASERTA+EKSPRESI+GEN+UNTUK+DETEKSI+ENDOTOKSIN+PENYAKIT%0D%0ABAKTERI+PATOGEN+PADA+IKAN&rft.creator=PETRA+TAMARA+%2C+SIMAMORA&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.subject=001+Ilmu+pengetahuan&rft.description=Lobster+hemolymph+is+explored+as+an+endotoxin+detector+and+an%0D%0Aalternative+antibacterial+test+due+to+its+sensitivity+to+lipopolysaccharides+(LPS)%2C%0D%0Asimilar+to+horseshoe+crabs.+Lobster+hemolymph+clots+quickly%2C+requiring+an%0D%0Aalternative+anticoagulant+like+fucoidan.+This+study+aimed+to+evaluate+the%0D%0Aanticoagulant+activity+of+fucoidan+from+brown+algae+using+the+Lee-White%0D%0Acoagulation+test%2C+agglutination+test%2C+and+gene+expression+analysis+in+lobster%0D%0Ahemolymph+for+detecting+endotoxins+from+bacteria+that+cause+diseases+in+fish.%0D%0AThis+study+employs+an+exploratory+method+by+screening+different+types+and+doses%0D%0Aof+anticoagulants+using+six+treatments%3A+control%2C+addition+of+10%25+aspirin%2C+10%25%0D%0AEDTA%2C+10%25+sodium+citrate%2C+heparin%2C+and+fucoidan+to+lobster+hemolymph.+The%0D%0Acoagulation+time+of+the+lobster+hemolymph+was+measured%2C+and+an+agglutination%0D%0Atest+was+observed+after+the+addition+of+fish+pathogenic+bacteria.+The+results+of+the%0D%0ALee-White+test+showed+that+the+hemolymph-to-fucoidan+ratios+of+2%3A3%2C+2%3A4%2C+and+2%3A5%0D%0Ainfluenced+coagulation.+The+agglutination+test+results+indicated+agglutination%0D%0Aformation+in+all+samples.+The+cycle+threshold+(CT)+values+for+gene+expression%0D%0Aanalysis+were+as+follows%3A+%CE%B2-Actin+1+at+34.9226%2C+%CE%B2-Actin+2+at+29.8296%2C+GPO+at%0D%0A42.042%2C+Lectin+1+at+41.937%2C+Lectin+2+at+42.1846%2C+LGBP+1+at+39.9524%2C+and+LGBP+2%0D%0Aat+35.8335.+In+conclusion%2C+fucoidan+can+be+used+as+an+anticoagulant+at+a+2%3A3+ratio%2C%0D%0Aand+lobster+hemolymph+has+the+potential+to+be+utilized+as+an+antibacterial+agent%0D%0Aagainst+fish+pathogens.+Additionally%2C+the+genes+%CE%B2-Actin%2C+LGBP%2C+Lectin%2C+and+GPO%0D%0Awere+successfully+expressed+in+the+hemolymph+of+the+sand+lobster+(Panulirus%0D%0Ahomarus).%0D%0AKeywords%3A+Agglutination+Test%2C+Coagulation+Test%2C+Endotoxins%2C+Fucoidan%2C+Gene%0D%0AExpression%2C+Spiny+Lobster%0D%0A%0D%0AHemolim+lobster+dieksplorasi+sebagai+detektor+endotoksin+dan+alternatif%0D%0Auji+antibakteri+karena+sensitif+terhadap+lipopolisakarida+(LPS)%2C+mirip+dengan%0D%0Akepiting+tapal+kuda.+Hemolim+lobster+cepat+menggumpal%2C+sehingga+dibutuhkan%0D%0Aalternatif+antikoagulan+seperti+fukoidan.+Tujuan+dari+penelitian+ini+adalah+untuk%0D%0Amenguji+aktivitas+antikoagulan+senyawa+fukoidan+dari+alga+coklat+menggunakan%0D%0Auji+koagulasi%2C+uji+aglutinasi+serta+ekspresi+gen+pada+hemolim+lobster+sebagai%0D%0Adeteksi+endotoksin+bakteri+penyebab+penyakit+ikan.+Penelitian+ini+menggunakan%0D%0Ametode+eksploratif+yaitu+dengan+skrining+jenis+dan+dosis+antikoagulan%0D%0Amenggunakan+6+perlakuan+yaitu+kontrol%2C+penambahan+aspirin+10%25%2C+EDTA+10%25%2C%0D%0Anatrium+sitrat+10%25%2C+heparin+dan+fukoidan+ke+hemolim+lobster+pasir+(Panulirus%0D%0Ahomarus)+lalu+dilakukan+perhitungan+lama+hemolim+lobster+menggumpal+dan%0D%0Adiamati+uji+agutinasi+yang+ditambah+bakteri+penyebab+penyakit+ikan.+Hasil%0D%0Apenelitian+uji+koagulasi+menunjukkan+bahwa+perbandingan+hemolim+dengan%0D%0Afukoidan+adalah+2%3A3%2C+2%3A4+dan+2%3A5%2C+untuk+hasil+uji+aglutinasi+menunjukkan%0D%0Asemuanya+mengalami+pembentukan+aglutinasi%2C+dan+nilai+CT+ekspresi+gen+sampel%0D%0A&rft.publisher=FAKULTAS+PERTANIAN&rft.date=2025-03-03&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F86277%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F86277%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F86277%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++PETRA+TAMARA+%2C+SIMAMORA++(2025)+AKTIVITAS+KOAGULASI+HEMOLIM+LOBSTER+PASIR+(Panulirus+homarus)+DENGAN+PENAMBAHAN+FUKOIDAN+DAN+UJI+AGLUTINASI+SERTA+EKSPRESI+GEN+UNTUK+DETEKSI+ENDOTOKSIN+PENYAKIT+BAKTERI+PATOGEN+PADA+IKAN.++FAKULTAS+PERTANIAN%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F86277%2F