?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=ANALISIS+mtDNA+BERDASARKAN+PROFIL+GEN+16S+rRNA+PADA%0D%0ALEBAH+TANPA+SENGAT+(Stingless+bee)+DI+KABUPATEN%0D%0A%0D%0ALAMPUNG+TIMUR&rft.creator=Aulia%2C+Imtitsal&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=570+Biologi&rft.description=Lebah+tanpa+sengat+memiliki+lebih+dari+600+spesies+di+seluruh+dunia.+Distribusi%0D%0Awilayah+persebaran+lebah+tanpa+sengat+di+dunia+dibagi+menjadi+3%2C+yaitu%0D%0ANeotropical%2C+Afrotropical%2C+dan+Australasian.+Sebanyak+46+spesies+dalam+10%0D%0Agenus+lebah+tanpa+sengat+yang+telah+tercatat+dari+Indonesia%2C+sebanyak+23+jenis%0D%0Alebah+tanpa+sengat+ditemukan+di+Sumatera.+Keragaman+lebah+tanpa+sengat+dan%0D%0Ahubungan+kekerabatan+dapat+diketahui+dengan+melakukan+identifikasi%0D%0Amorfologi.+Penggunaan+analisis+morfologi+memiliki+kelemahan+seperti%0D%0Arendahnya+nilai+konsistensi+yang+menunjukkan+hubungan+filogenetik+pada%0D%0Atingkat+variasi+spesies.+Analisis+filogenetik+penting+dilakukan+untuk+mengetahui%0D%0Aberbagai+keragaman+lebah+tanpa+sengat+yang+dapat+menjadi+salah+satu+upaya%0D%0Akonservasi+lebah+tanpa+sengat+di+Kabupaten+Lampung+Timur%2C+sehingga+perlu%0D%0Adilakukan+analisis+molekuler+keragaman+lebah+tanpa+sengat+menggunakan%0D%0ADNA+mitokondria+(mtDNA)+pada+lebah.+Gen+16S+rRNA+merupakan+gen%0D%0Apenyandi+mtDNA+yang+paling+informatif+untuk+filogenetik+terutama+pada+ordo%0D%0AHymenoptera.+Penelitian+ini+bertujuan+untuk+deteksi+keragaman+spesies+lebah%0D%0Atanpa+sengat+secara+molekuler+dengan+profil+gen+16S+rRNA+di+Kabupaten%0D%0ALampung+Timur.+Metode+yang+digunakan+adalah+isolasi+DNA%2C+amplifikasi+gen%0D%0ADNA%2C+elektroforesis+dan+visualisasi%2C+sekuensing+serta+analisis+hasil+sekuensing%0D%0Adengan+perangkat+lunak+menggunakan+Basic+Local+Alignment+Search+Tool%0D%0A(BLAST)+dan+konstruksi+pohon+filogenetik+menggunakan+Molecular+Evolution%0D%0AGenetic+Analysis+(MEGA11)+dengan+metode+maximum+likelihood+serta+metode%0D%0Abootsrap+sebanyak+1000+pengulangan.+Berdasarkan+penelitian+yang+telah%0D%0Adilakukan+didapatkan+hasil+bahwa+ketiga+sampel+lebah+tanpa+sengat+memiliki%0D%0Ahubungan+kekerabatan+yang+dekat+dengan+spesies+Heterotrigona+itama+dengan%0D%0Anilai+jarak+genetik+0%2C0176+dengan+homologi+97%2C95%25+menunjukkan+tingkat%0D%0Akeragaman+genetic+yang+rendah.%0D%0A%0D%0AKata+kunci%3A+Lebah+tanpa+sengat%2C+Analisis+Molekuler%2C+Gen+16S+rRNA%2C%0D%0A%0D%0AFilogenetik.%0D%0A%0D%0AStingless+bees+have+more+than+600+species+worldwide.+The+distribution+area%0D%0Aof+stingless+bees+in+the+world+is+divided+into+3%2C+Neotropical%2C+Afrotropical%2C%0D%0Aand+Australasian.+A+total+of+46+species+in+10+genus+of+stingless+bees+have%0D%0Abeen+recorded+in+Indonesia%2C+23+species+of+stingless+bees+were+found+in%0D%0ASumatra.+The+diversity+of+stingless+bees+and+the+kinship+of+each+group+of%0D%0Abees+known+by+conducting+morphological+identification.+However%2C+the+use%0D%0Aof+morphological+characteristic+has+shortcomings+such+as+low+consistency%0D%0Avalues+for+phylogenetic+relationships+at+the+species+variation+level.%0D%0APhylogenetic+analysis+is+important+to+determine+the+diversity+of+stingless%0D%0Abees+which+can+be+one+of+the+conservation+efforts+of+stingless+bees+in+East%0D%0ALampung.+It+is+necessary+to+conduct+molecular+analysis+of+stingless+bee%0D%0Adiversity+using+mitochondrial+DNA+(mtDNA)+in+bees.+The+16S+rRNA+gene+is%0D%0Athe+most+informative+mtDNA+encoding+gene+for+phylogenetics%2C+especially%0D%0Ain+the+order+Hymenoptera.+This+study+aims+to+detect+the+diversity+of%0D%0Astingless+bee+species+molecularly+with+16S+rRNA+gene+profiles+in+East%0D%0ALampung+Regency.+The+methods+used+were+DNA+isolation%2C+DNA+gene%0D%0Aamplification%2C+electrophoresis+and+visualization%2C+sequencing+and+analysis+of%0D%0Asequencing+results+with+software+using+the+Basic+Local+Alignment+Search%0D%0ATool+(BLAST)+and+phylogenetic+tree+construction+using+Molecular%0D%0AEvolution+Genetic+Analysis+(MEGA+11)+with+the+maximum+like+lihood%0D%0Amethod+and+the+boots+method.+Based+on+the+research+that+has+been+done%2C%0D%0Athe+results+show+that+the+three+stingless+bee+samples+have+a+close+kinship%0D%0Awith+the+Heterotrigona+itama+species+with+a+genetic+distance+value+of%0D%0A0.0176+with+97.95%25+homology+showing+a+low+level+of+genetic+diversity.%0D%0AKeywords%3A+Stingless+bee%2C+Molecular+Analysis%2C+16S+rRNA+gene%2C%0D%0A%0D%0APhylogenetic.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-06-28&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F86604%2F1%2FABSTRAK%2520SKRIPSI_AULIA%2520IMTITSAL%2520-%2520Aulia%2520Imtitsal.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F86604%2F2%2FSKRIPSI%2520TANPA%2520LAMPIRAN%2520ISI_AULIA%2520IMTITSAL%2520-%2520Aulia%2520Imtitsal.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F86604%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN_AULIA%2520IMTITSAL%2520-%2520Aulia%2520Imtitsal.pdf&rft.identifier=++Aulia%2C+Imtitsal++(2024)+ANALISIS+mtDNA+BERDASARKAN+PROFIL+GEN+16S+rRNA+PADA+LEBAH+TANPA+SENGAT+(Stingless+bee)+DI+KABUPATEN+LAMPUNG+TIMUR.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F86604%2F