@misc{eprints86641,
           month = {Mei},
           title = {ANALISIS PROFIL PROTEIN DAN UJI PENENTUAN KONSENTRASI
PROTEIN DAUN TANAMAN CASSAVA (Manihot esculenta Crantz)
SETELAH DIINDUKSI DENGAN ASAM SALISILAT},
          author = { Nur Afifah 	 	Salma},
         address = {UNIVERSITAS LAMPUNG},
       publisher = {FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM },
            year = {2024},
             url = {http://digilib.unila.ac.id/86641/},
        abstract = {Cassava (Manihot esculenta Crantz) merupakan tanaman penting di Indonesia
sebagai sumber karbohidrat. Cassava sering kali mendapatkan gangguan patogen
salah satunya yaitu penyakit layu fusarium. Pengendalian penyakit layu fusarium
dapat dilakukan dengan penggunaan kultivar unggul yang resisten melalui
pengimbasan asam salisilat. Tujuan penelitian ini adalah untuk mengetahui nilai
konsentrasi protein dan menganalisis profil protein pada tanaman cassava antara
cassava yang telah diberi pengimbasan asam salisilat dengan kontrol. Penelitian ini
menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor, yaitu
penambahan asam salisilat yang dibagi atas 5 taraf konsentrasi, yaitu 0 ppm, 80
ppm, 100 ppm, 120 ppm, dan 140 ppm. Masing ? masing dari konsentrasi tersebut
dilakukan pengulangan sebanyak 5 kali, dan pada setiap ulangan terdiri atas 1
tanaman cassava. Data penelitian berupa data kualitatif dan data kuantitatif. Data
kualitatif disajikan dalam bentuk deskriptif komparatif yang didukung dengan foto
sedangkan data kuantitaif dianalisis menggunakan Statistical Package forthe Social
Sciences (SPSS) dengan metode Analysis of Variance (ANOVA). Apabila terdapat
beda nyata dilanjutkan dengan Uji Duncan's Multiple Range Test (DMRT) 5\%.
Hasil penelitian menunjukkan beda nyata pada konsentrasi asam salisilat 80 ppm,
100 ppm, 120 ppm, dan 140 ppm setelah dibandingkan dengan kontrol. Taraf
konsentrasi protein 0 ppm sampai 140 ppm berturut turut adalah 0,370 mg/2 {\ensuremath{\mu}}l,
0,372 mg/2{\ensuremath{\mu}}l, 0,377 mg/2 {\ensuremath{\mu}}l, 0,373 mg/2 {\ensuremath{\mu}}l, dan 0, 369 mg/2 {\ensuremath{\mu}}l. Serta terdapat pita
protein hilang (missing band) yaitu pita pada berat molekul 115 kDa (perlakuan
konsentrasi asam salisilat 140 ppm), dan juga ada kemunculan satu pitabaru (new
band) yaitu pada berat molekul 85 kDa (perlakuan konsentrasi asam salisilat 100
ppm).

Kata kunci : Asam salisilat, Cassava, Profil protein, In vivo, Induced resistance
Cassava (Manihot esculenta Crantz) is an important plant in Indonesia as a source
of carbohydrates. Cassava often suffers from pathogenic disorders, one of which is
fusarium wilt. Control of fusarium wilt disease can be done by using superior
cultivars that are resistant through the use of salicylic acid. The aim of this research
was to determine the protein concentration value and analyze the protein profile in
cassava plants between cassava that had been treated with salicylic acid and the
control. This research used a Completely Randomized Design (CRD) with one
factor, namely the addition of salicylic acid which was divided into 5 concentration
levels, namely 0 ppm, 80 ppm, 100 ppm, 120 ppm, and 140 ppm. Each of these
concentrations was repeated 5 times, and each repetition consisted of 1 cassava
plant. Research data consists of qualitative data and quantitative data. Qualitative
data is presented in comparative descriptive form supported by photos, while
quantitative data is analyzed using the Statistical Package for the Social Sciences
(SPSS) with the Analysis of Variance (ANOVA) method. If there is a significant
difference, continue with Duncan's Multiple Range Test (DMRT) 5\%. The results
of the study showed a significant difference in salicylic acid concentrations of 80
ppm, 100 ppm, 120 ppm, and 140 ppm after being compared with the control. The
protein concentration levels from 0 ppm to 140 ppm are respectively 0.370 mg/2
{\ensuremath{\mu}}l, 0.372 mg/2 {\ensuremath{\mu}}l, 0.377 mg/2 {\ensuremath{\mu}}l, 0.373 mg/2 {\ensuremath{\mu}}l, and 0.369 mg/2 {\ensuremath{\mu}}l. There was also
a missing protein band, namely a band at a molecular weight of 115 kDa (treatment
with a salicylic acid concentration of 140 ppm), and there was also the appearance
of a new band, namely a molecular weight of 85 kDa (treatment with a salicylic
acid concentration of 100 ppm).

Keywords: Salicylic acid, Cassava, Protein profile, In vivo, Induced resistance}
}