%A Pirdaus Purna 
%T OPTIMASI DAN VALIDASI METODE DISPERSIVE SOLID PHASE
EXTRACTION (DSPE) MENGGUNAKAN GRAPHENE OXIDE DARI
LIMBAH KULIT SINGKONG (Manihot Utilissima) UNTUK PENENTUAN

RESIDU ANTIBIOTIK TETRASIKLIN
%X Meningkatnya penggunaan antibiotik tetrasiklin di bidang medis, peternakan dan
pertanian dapat menyebabkan timbulnya masalah baru yang serius yaitu
munculnya residu antibiotik di lingkungan perairan. Pencemaran residu tetrasiklin
mengakibatkan resistensi gen bakteri dan terganggunya keseimbang organisme
akuatik. Penelitian ini, telah dilakukan pembuatan graphene oxide dari limbah
kulit singkong sebagai adsorben untuk penentuan residu tetrasiklin dengan teknik
dispersive solid phase extraction (DSPE). Pembuatan graphene oxide dilakukan
menggunakan metode Hummers termodifikasi. Karakterisasi graphene oxide

dilakukan menggunakan spektrofotometer FTIR, spektometer XRD dan SEM-
EDX. Optimasi DSPE menggunakan graphene oxide kulit singkong dilakukan

dengan lima variasi, yaitu variasi konsentrasi tetrasiklin, pH, massa adsorben,
waktu kontak dan pelarut desorpsi. Hasil penelitian menunjukkan bahwa kondisi
optimum menggunakan graphene oxide sebanyak 20 mg dengan konsentrasi
tetrasiklin 10 ppm pada kondisi pH 5 dan waktu kontak selama 10 menit dengan
tingkat adsorpsi sebesar 66,03% serta menggunakan pelarut metanol untuk proses
desorpsi dengan tingkat desorpsi sebesar 87,75%. Validasi metode menunjukan
hasil yang baik dengan memenuhi batas keberterimaan setiap parameter uji.
Parameter linieritas (r) 0,9997, presisi (RSD) 5,47% dan akurasi (recovery) 81,40
? 93,66%. Batas deteksi (LoD) 1,769 mg/L dan dan batas kuantifikasi (LoQ)
5,895 mg/L .

Kata kunci: Antibiotik tetrasiklin, DSPE, kulit singkong, graphene oxide,

validasi metode

ABSTRACT

OPTIMIZATION AND VALIDATION OF THE DISPERSIVE SOLID
PHASE EXTRACTION (DSPE) METHOD USING GRAPHENE OXIDE
FROM CASSAVA PEEL WASTE (Manihot Utilissima) FOR
DETERMINING TETRACYCLINE ANTIBIOTIC RESIDUES

By

Purna Pirdaus

The increasing use of tetracycline antibiotics in the medical, livestock and
agricultural fields can cause the emergence of a serious new problem, namely the
emergence of antibiotic residues in the aquatic environment. Tetracycline residue
contamination results in bacterial resistance genes and disrupts the balance of
aquatic organisms. In this research, graphene oxide was made from cassava peel
waste as an adsorbent to determine tetracycline residue using the dispersive solid
phase extraction (DSPE) technique. Graphene oxide was made using a modified
Hummers method. Characterization of graphene oxide was carried out using an
FTIR spectrophotometer, XRD spectrometer and SEM-EDX. Optimization of
DSPE using cassava peel graphene oxide was carried out with five variations,
namely variations in concentration, pH, mass, contact time and desorption solvent.
The research results show that the optimum conditions were using 20 mg
graphene oxide with an adsorbate concentration of 10 ppm at pH 5 and a contact
time of 10 minutes with an adsorption rate of 66.03% and using methanol solvent
for the desorption process with a desorption rate of 87.75%. Validation of the
method showed good results by meeting the acceptance limits for each test
parameter. Linearity parameters (r) 0.9997; precision (RSD) 5.47% and accuracy
(recovery) 81.40 ? 93.66%. The limit of detection (LoD) and limit of
quantification (LoQ) were found to be 1.769 and 5.895 mg/L respectively.

Key words: Tetracycline antibiotics, DSPE, cassava peel, graphene oxide,

method validation
%D 2024
%I UNIVERSITAS LAMPUNG
%L eprints86969