creators_name: Ammanda Sadiva,   Ajeng  Pawesti
creators_id: 2117061031   
type: other
datestamp: 2025-06-11 06:27:26
lastmod: 2025-06-11 06:27:26
metadata_visibility: show
title: Potensi Antibakteri Ekstrak Metanol Gracilaria sp. terhadap Escherichia coli dengan Metode Difusi Cakram 
ispublished: pub
subjects: 500
subjects: 570
full_text_status: restricted
abstract: Penggunaan sumber daya laut sebagai alternatif bahan  antibakteri alami semakin berkembang  dalam  beberapa  tahun  terakhir.  Gracilaria  sp.,  sejenis  alga  merah, diketahui  memiliki  kandungan  metabolit  sekunder  yang  berpotensi  sebagai  agen antibakteri,  khususnya  terhadap  Escherichia  coli.  Penelitian  ini  bertujuan  untuk menentukan potensi antibakteri ekstrak metanol Gracilaria sp. terhadap    E. coli menggunakan  metode  jenis  eksperimental  dengan  Rancangan  Acak  Lengkap (RAL) dengan analisis data One-Way ANOVA dan dilanjutkan dengan Uji Tukey. Larutan  stok  ekstrak  dibuat  dengan  konsentrasi  100  mg/mL  dan  diuji  pada konsentrasi  0,05%,  1%,  5%,  dan  10%  dengan  kontrol  positif  ciprofloxacin  dan kontrol  negatif  akuades  steril.  Pengujian dilakukan  dalam  tiga  kali  pengulangan. Hasil penelitian menunjukkan bahwa ekstrak metanol Gracilaria sp. menghasilkan zona hambat yang terbentuk pada konsentrasi tertinggi 10% dan 5% dengan rata- rata hanya mencapai (0,075 mm). Dengan demikian, ekstrak metanol Gracilaria sp. memiliki  potensi  antibakteri  yang  lemah  terhadap  Escherichia  coli,  karena  zona hambat yang terbentuk jauh lebih kecil dibandingkan kontrol positif yang mencapai 1,7 mm. 
Kata kunci: Gracilaria sp., antibakteri, Escherichia coli, difusi cakram, ekstrak metanol. 

The use of marine resources as an alternative source of natural antibacterial agents has been increasingly developed in recent years. Gracilaria sp., a type of red algae, is  known  to  contain  biologically  active  compounds  that  have  potential  as antibacterial  agents,  particularly  against  Escherichia  coli.  This  study  aimed  to determine  the  antibacterial  potential  of  the  methanolic  extract  of  Gracilaria  sp. against E. coli using an experimental method based on a Completely Randomized Design (CRD). Data were analyzed using One-Way ANOVA followed by Tukey’s test. A stock solution of the extract was prepared at a concentration of 100 mg/mL and tested at concentrations of 0.05%, 1%, 5%, and 10%, with ciprofloxacin as the positive  control  and  sterile  distilled  water  as  the  negative  control.  The  test  was conducted  in  three  replicates.  The  results  showed  that  the  methanolic  extract  of Gracilaria sp. produced an inhibition zone at the highest concentrations (10% and 5%) with an average diameter of only 0.075 mm. Therefore, the methanolic extract of Gracilaria sp. has weak antibacterial potential against  Escherichia coli, as the inhibition  zone  formed  is  significantly  smaller  than  that  of  the  positive  control, which reached 1.7 mm. 
Keywords:  Gracilaria  sp.,  antibacterial,  Escherichia  coli,  disk  diffusion, methanolic extract. 
date: 2025-05-21
date_type: published
publisher: FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
place_of_pub: UNIVERSITAS LAMPUNG
citation:   Ammanda Sadiva, Ajeng Pawesti  (2025) Potensi Antibakteri Ekstrak Metanol Gracilaria sp. terhadap Escherichia coli dengan Metode Difusi Cakram.  FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM, UNIVERSITAS LAMPUNG.     
document_url: http://digilib.unila.ac.id/88304/1/ABSTRAK.pdf
document_url: http://digilib.unila.ac.id/88304/2/SKRIPSI%20FULL.pdf
document_url: http://digilib.unila.ac.id/88304/3/SKRIPSI%20TANPA%20BAB%20PEMBAHASAN.pdf