Digital Library: No conditions. Results ordered -Date Deposited. 2024-03-29T09:05:28ZEPrintshttp://digilib.unila.ac.id/images/sitelogo.pnghttp://digilib.unila.ac.id/2015-11-27T08:08:43Z2015-11-27T08:08:43Zhttp://digilib.unila.ac.id/id/eprint/14878This item is in the repository with the URL: http://digilib.unila.ac.id/id/eprint/148782015-11-27T08:08:43ZUJI IN VITRO BEBERAPA ISOLAT Trichoderma spp. DAN UJI
EFEKTIVITAS Trichoderma harzianum SERTA BAHAN
ORGANIK DALAM MENGENDALIKAN PENYAKIT
BUSUK PANGKAL BATANG LADA
DI LAPANGANKendala serius dalam budidaya tanaman lada adalah penyakit busuk pangkal batang
lada (BPBL) yang disebabkan oleh jamur Phytophthora capsici. Penelitian ini
bertujuan untuk mengetahui keefektifan isolat Trichoderma harzianum yang
dikombinasikan dengan bahan organik dalam mengendalikan penyakit Busuk Pangkal
Batang Lada di lapangan. Hipotesis yang diajukan dalam penelitian ini adalah (1) isolat
Trichoderma sp. dapat menekan pertumbuhan Phytophthora capsici secara in vitro; (2)
kemampuan Trichoderma dalam menekan pertumbuhan Phytophthora capsici berbedabeda
antar-isolat; (3) kombinasi antara isolat Trichoderma sp. terpilih dan bahan
organik dapat menekan perkembangan penyakit BPBL di lapangan; (4) jenis bahan
organik yang berbeda mempunyai kemampuan yang berbeda secara tidak langsung
dalam menekan perkembangan penyakit. Untuk tes in vitro digunakan tujuh isolat
Trichoderma spp., yaitu dua isolat dari tanah kebun percobaan di Cahaya Negeri dan
lima isolat dari koleksi Laboratorium Proteksi Tanaman FP UNILA. Di lapangan,
perlakuan disusun dalam rancangan acak kelompok dengan sepuluh ulangan. Data hasil
pengamatan dianalisis dengan sidik ragam dan perbedaan nilai tengah antarperlakuan
diuji dengan uji BNT dengan taraf nyata 5%. Hasil penelitian menunjukkan isolat T3 M
(Trichoderma harzianum) memiliki daya antagonis lebih tinggi dibandingkan isolat T1
M, T2 M , T1, T3, Tv dan Tk. Perlakuan jamur Trichoderma harzianum dan bahan
organik tidak menurunkan keterjadian penyakit. Selain itu, perlakuan jamur
Trichoderma harzianum dan bahan organik tersebut juga meningkatkan kepadatan
jamur Trichoderma harzianum dalam tanah di lapangan.
Kata Kunci: Bahan Organik, busuk pangkal batang lada, Phytophthora capsici,
Trichoderma
Abstrak Bahasa Inggris
Serious problem in the crop cultivation of black pepper is foot rot caused by the fungus
Phytophthora capsici. The objective of this research was to determine the effectiveness
of isolates of Trichoderma harzianum combined with organic matter to controll foot rot
paper disease in the field. The hypothesis of this research were (1) isolates of
Trichoderma sp. can suppress the growth of Phytophthora capsici in vitro; (2) the
ability of Trichoderma in suppressing the growth of Phytophthora capsici differed
between isolates; (3) the combination of isolates of Trichoderma sp. and organic matter
can suppress foot rot progression in the field; (4) a different type of organic matter has
different capabilities indirectly to suppress progression of the disease. For in vitro test,
total of seven Trichoderma isolates (two were isolates from Cahaya Negeri black
pepper field and five were from cultur collection) were used in this experiment. In the
field, treatments arranged in a randomized block design with ten replications. The data
were analyzed by analysis of variance and differences followed by LSD test with
significance level at 5%. The results showed that isolate T3 M (Trichoderma
harzianum) has a higher power than isolate antagonists T2 M, T1, T3, T1 M, Tv and Tk.
Treatment Trichoderma harzianum and organic matter did not decrease the occurrence
of the disease. In addition, this treatment increased the density of Trichoderma
harzianum in the soil in the field.
Keywords: Organic matter, foot rot pepper, Phytophthora capsici, Trichoderma1114121034 ARIS NURHIDAYATmas.sares369@gmail.com