0514111042, ELMUNAH WAHIDAH (2012) PATOGENISITAS Aeromonas salmonicida PADA PATIN SIAM (Pangasius hypophthalmus). UNSPECIFIED.
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Abstract
Abstrak Aeromonas salmonicida merupakan bakteri pathogen pada ikan yang memiliki kisaran inang yang luas. Tujuan penelitian ini adalah untuk mengamati dan mengetahui patogenisitas A. salmonicida pada ikan patin siam. Isolate bakteri A. salmonicida dikoleksi dari Stasiun Karantina Ikan Panjang. Ada tiga parameter yang diamati untuk mengetahui patogenisitas A. salmonicida pada penelitian ini yaitu, LD50, uji hemaglutinasi dan uji Postulat Koch. LD50 diambil dari data sekunder pada penelitian sebelumnya oleh Supriyadi (2010). Uji hemaglutinasi dilakukan dengan mengacu pada metode standar uji hemaglutinasi VIRAPUR (2011) dengan sedikit modifikasi. Uji Postulat Koch dilakukan dengan menginfeksikan isolat A. salmonicida dari ikan yang diinfeksi sebelumnya pada 10 ekor ikan patin dan diamati gejala sakit dan kematian selama 2 minggu. Hasil penelitian menunjukkan LD50 A. salmonicida pada ikan patin siam adalah 107cfu/mL. Uji hemaglutinasi menunjukkan adanya reaksi aglutinasi eritrosit ikan patin pada pengenceran 21. Uji Postulat Koch menunjukkan adanya kematian pada ikan patin sebanyak 2 ekor tanpa diawali dengan gejala klinis. Hasil penelitian tersebut menunjukkan bahwa bakteri A. salmonicida memiliki patogenisitas yang relatif rendah pada ikan patin siam. Kata kunci : A. salmonicida, Hemaglutinasi, LD50, Patin siam, Postulat Koch Abstract Aeromonas salmonicida is pathogenic bacteria in fish that has wide range host. The objective of this research was to know pathogenicity of A. salmonicida on striped catfish. A. salmonicida isolate was collected from Panjang fish quarantine station. There were three parameters observed to detect pathogenicity of A. salmonicida in this research : LD50, hemagglutination test and Koch Postulate test. LD50 was taken from the secondary data in a previous research by Supriyadi (2010). Hemagglutination test was referred to standard method of VIRAPUR hemagglutination test (2011) with minor modification. Koch Postulate test was done by infecting A. salmonicida from a previously infected fish into ten striped catfish. The symptom of disease and mortality was observed daily in two weeks after infection. The results showed that LD50 A. salmonicida on striped catfish was 107 cfu/mL. The hemagglutination test showed that there was agglutination reaction of erythrocyte on striped catfish in 2-1 dilution. Koch Postulate test indicated that there were 2 fish died without any initiated clinical symptom. These resulted that A. salmonicida isolate of this research has low pathogenicity on striped catfish.. Key words : A. salmonicida, LD50, hemagglutination, Koch Postulat, striped catfish.
| Item Type: | Other |
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| Subjects: | A General Works = Karya Karya Umum |
| Divisions: | Fakultas Pertanian > Prodi Budidaya Perairan |
| Depositing User: | IC-STAR . 2015 |
| Date Deposited: | 07 Sep 2015 04:03 |
| Last Modified: | 11 Sep 2015 07:00 |
| URI: | http://digilib.unila.ac.id/id/eprint/12303 |
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