Anggraini Yulistia, 1327011022 (2015) BIOAKTIVITAS ASAM CIS-EIKOS-9-ENOAT DARI Oscillatoria sp. TERHADAP E. coli RESISTEN CHLORAMPHENICOL. Masters thesis, Universitas Lampung.
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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)
ABSTRAK BIOAKTIVITAS ASAM CIS-EIKOS-9-ENOAT DARI Oscillatoria sp. TERHADAP E. coli RESISTEN CHLORAMPHENICOL Oleh Yulistia Anggraini Oscillatoria sp. dapat menjadi sumber potensial penghasil senyawa metabolit bioaktif. Hasil uji bioaktivitas awal menunjukkan bahwa fraksi polar dari Oscillatoria sp berpotensi sebagai senyawa antibakteri terhadap E. coli yang telah resisten terhadap chloramphenicol. Isolasi berdasarkan panduan uji bioaktivitas memandu pada isolasi senyawa asam cis-eikos-9-enoat (C20H38O2). Spektrum FTIR menunjukkan karakteristik puncak serapan vibrasi gugus O-H pada daerah 3434,6 dan 2669,0 cm-1; gugus alkil rantai panjang di daerah 2919,7 dan 2849,3 cm-1; dan gugus karbonil di daerah 1702,8 cm-1. Spektrum 13C NMR menunjukkan adanya signal proton di δC 178,7 ppm (karbon karbonil), δC 14,9 dan 22,9-31,7 ppm (karbon sp3), serta δC 129,7 dan 130,0 ppm (karbon sp2). Spektrum 1H NMR menunjukkan adanya signal proton pada δH 0,8 ppm (-CH3); δH 1,3-2,4 ppm (-CH2-), dan δH 5,4 ppm (proton pada alkena). Karakteristik ikatan rangkap asam cis-eikos-9-enoat ditunjukkan oleh adanya puncak serapan multiplet pada δH 5,4 ppm dengan J 5,2; 6,1; dan 6,4 Hz. Hasil uji bioaktivitas menunjukkan bahwa asam cis-eikos-9-enoat memiliki potensi sebagai antibakteri terhadap E. coli resisten chloramphenicol dengan daya hambat sebesar 7 mm pada dosis 100 μg. Kata kunci : Oscillatoria sp., antibakteri, E. coli resisten, chloramphenicol, asam cis-eikos-9-enoat ABSTRACT BIOACTIVITY OF CIS-ICOS-9-ENOIC ACID FROM Oscillatoria sp. AGAINST E. coli RESISTANCE TO CHLORAMPHENICOL By Yulistia Anggraini Oscillatoria sp. could be a potential sources for bioactive metabolite producer. The bioactivity preliminary test showed that the polar fraction of biomass extract from Oscillatoria sp. has a potency as an antibacterial agent against E. coli resistance to chloramphenicol. The bioassay guided separation leads to isolate cis-icos-9-enoic acid (C20H38O2). The FTIR spectrum showed the characteristic vibration peaks of O-H group at 3434.6-2669.0 cm-1 (broadening), alkyl group at 2919.7 and 2849.3 cm-1 (Sharp), and C=O group at 1702.8 cm-1. The 13C NMR spectrum showed the proton signal at δC 178.7 ppm of carbonil, δC 14.9; 22.9-31.7 ppm of carbon sp3, and δC 129.7 and 130.0 ppm of carbon sp2. The 1H NMR spectrum showed the proton signal at δH 0.8 ppm (-CH3), δH 1.3-2.4 ppm (-CH2), and δH 5.4 ppm (=CH). The double bond characteristic of cis-icos-9-enoic acid is showed by 1H NMR at δH 5.4 ppm (m) with J 5.2; 6.1; and 6.4 Hz. The result of bioactivity test showed that cis-icos-9-enoic acid has antibacterial potency to against E. coli resistance to chloramphenicol with inhibition zone value 7 mm at 100 μg of dose. Key words: Oscillatoria sp., antibacterial, E. coli resistance, chloramphenicol, cis-icos-9-enoic acid
| Jenis Karya Akhir: | Tesis (Masters) |
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| Subyek: | |
| Program Studi: | FAKULTAS MIPA > Prodi Magister Ilmu Kimia |
| Pengguna Deposit: | 2764869 . Digilib |
| Date Deposited: | 05 Jan 2016 03:16 |
| Terakhir diubah: | 05 Jan 2016 03:16 |
| URI: | http://digilib.unila.ac.id/id/eprint/16566 |
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