STUDY OF ALKALI AND ENZYMATIC PRETREATMENTS ON SUGARCANE BAGASSE TO PRODUCE REDUCED SUGAR

0924051018, REFIARIOEN (2012) STUDY OF ALKALI AND ENZYMATIC PRETREATMENTS ON SUGARCANE BAGASSE TO PRODUCE REDUCED SUGAR. Digital Library.

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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)

Abstrak Sugarcane bagasse is one of solid waste from sugar factories. It contains lignocelluloses that can be hydrolyzed into reduced sugar. Objectives of this study were to find out (1) the best condition (alkali concentration, lignin degradation time and temperature) of bagasse lignin degradation, and (2) the best substrate concentration and enzymatic incubation time for hydrolyzing baggase holocelluloses into reduced sugar. This study was performed into two phases, namely lignin degradation and enzymatic hydrolysis. During the former phase, sugarcane bagasse was immersed in alkali (NaOH, H2O2) solution at a variety of concentrations for a variety of times and temperatures. After degradation, samples were taken and analyzed for degree of lignin degradation. During the latter phases, 3.75% - 6.25% baggase lignocelluloses were hydrolyzed with 10 FPU cellulase enzyme for 0 – 24 hours at pH 4.8, 50oC, and 200 rpm. After hydrolyzation process, samples were taken and analyzed for determining their reduced sugar contents. Collected data were analyzed descriptively and presented graphycally. Results of this study shown that (1) the best condition for lignin degradation (> 99% lignin degraded) was immersion of the sugarcane baggase into 1.0 M NaOH solution at 121 oC for 15 minutes, and (2) the best treatment for sugarcane bagasse enzymataic hydrolysis was a substrate concentration of 6.25% and a hydrolysis time of 18 hours. The hydrolysis treatment yielded a reduced sugar content of 19.29g/L and a bagasse carbon conversion of 12,4%. Key words: alkali, bagasse, pretreatment, NaOH, enzymatic hydrolysis.

Jenis Karya Akhir: Artikel
Subyek:
Pengguna Deposit: tik 12 . Digilib
Date Deposited: 13 Jan 2016 06:49
Terakhir diubah: 13 Jan 2016 06:49
URI: http://digilib.unila.ac.id/id/eprint/17337

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