Atika Yumna Sabita, - (2025) APLIKASI PORTABLE LED-BASED FLUORESCENCE SPECTROSCOPY DAN KEMOMETRIKA UNTUK AUTENTIKASI MADU LEBAH TANPA SENGAT (Tetragonula laeviceps). FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.
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Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)
Madu Tetragonula laeviceps merupakan jenis madu dari lebah madu tanpa sengat yang bermanfaat bagi kesehatan. Namun, produksi yang tidak seimbang dan tingginya permintaan menyebabkan maraknya pemalsuan, umumnya dengan mencampurkan madu asli dengan sirup beras merah (rice malt syrup). Penelitian ini bertujuan mengautentikasi madu Tetragonula laeviceps menggunakan spektroskopi fluoresensi portabel berbasis LED dan analisis kemometrika. Pengujian dilakukan dengan tiga sampel yaitu madu murni Tetragonula laeviceps nektar Calliandra callothyrsus (TL), madu yang dicampur dengan sirup beras (TLC) dalam rasio 10-60%, serta sirup beras murni (SB). Data spektra dianalisis menggunakan metode Soft Independent Modeling of Class Analogy (SIMCA) dengan beberapa teknik pretreatment Smoothing Moving Average (SMA) dan Multiplicative Scatter Correction (MSC). Hasil menunjukkan perbedaan nilai fluoresensi antara madu murni, campuran, dan sirup beras, khususnya pada panjang gelombang 484,5 nm. Model terbaik diperoleh dengan pretreatment MSC + SMA 5 segment, yang menghasilkan akurasi klasifikasi dan spesifisitas 100%, sensitivitas 0%, serta error 0%. Penelitian ini membuktikan bahwa spektroskopi fluoresensi portabel merupakan metode yang efektif untuk autentikasi madu Tetragonula laeviceps, mampu membedakan produk madu asli dan campuran, serta membantu mendeteksi pemalsuan secara cepat, sehingga memberikan perlindungan lebih baik bagi konsumen. Kata kunci: Madu Tetragonula laeviceps, Spektroskopi fluoresensi, Kemometrika, Autentikasi, SIMCA Tetragonula laeviceps honey, produced by stingless bees, is known for its health benefits. However, the imbalance between limited production and high demand has led to increasing cases of adulteration, commonly by mixing genuine honey with red rice syrup (rice malt syrup). This study aims to authenticate Tetragonula laeviceps honey using portable LED-based fluorescence spectroscopy combined with chemometric analysis. The calibration was conducted on three types of samples: pure Tetragonula laeviceps honey from Calliandra callothyrsus nectar (TL), honey adulterated with rice syrup (TLC) in ratios ranging from 10% to 60%, and pure rice syrup (SB). Spectral data were analyzed using the Soft Independent Modeling of Class Analogy (SIMCA) method, with several preprocessing techniques including Smoothing Moving Average (SMA) and Multiplicative Scatter Correction (MSC). The results showed clear differences in fluorescence intensity among pure honey, adulterated honey, and rice syrup, particularly at a wavelength of 484.5 nm. The best classification model was obtained using the MSC + SMA (5-segment) preprocessing combination, which achieved 100% classification accuracy and specificity, 0% sensitivity, and 0% error rate. This research demonstrates that portable fluorescence spectroscopy is an effective method for authenticating Tetragonula laeviceps honey. It can distinguish between pure and adulterated products rapidly and non-destructively, thus offering enhanced consumer protection and supporting quality assurance in honey production. Keywords: Tetragonula laeviceps honey, Fluorescence spectroscopy, Chemometrics, Authentication, SIMCA.
| Jenis Karya Akhir: | Skripsi |
|---|---|
| Subyek: | 600 Teknologi (ilmu terapan) 600 Teknologi (ilmu terapan) > 630 Pertanian dan teknologi yang berkaitan |
| Program Studi: | FAKULTAS PERTANIAN (FP) & PASCASERJANA > Prodi S1 Teknik Pertanian |
| Pengguna Deposit: | UPT . Ery Elyasari |
| Date Deposited: | 30 Oct 2025 01:22 |
| Terakhir diubah: | 30 Oct 2025 01:22 |
| URI: | http://digilib.unila.ac.id/id/eprint/92349 |
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