AKTIVITAS ANTI FUNGI EKSTRAK KULIT HUJAN EMAS (Senna multijuga) TERHADAP JAMUR Ganoderma boninense PADA SAWIT SECARA IN VITRO DAN IN SILICO

Diah , Pangastuti Rahayu (2025) AKTIVITAS ANTI FUNGI EKSTRAK KULIT HUJAN EMAS (Senna multijuga) TERHADAP JAMUR Ganoderma boninense PADA SAWIT SECARA IN VITRO DAN IN SILICO. Masters thesis, UNIVERSITAS LAMPUNG .

[img]
Preview
 File PDF
ABSTRAK-3-4 - Diah pangastuti Rahayu.pdf
Download (222Kb) | Preview
[img] File PDF
FULL-halaman - Diah pangastuti Rahayu.pdf
Restricted to Hanya staf
Download (3229Kb) | Minta salinan
[img]
Preview
 File PDF
FULL-tanpa pembahasan - Diah pangastuti Rahayu.pdf
Download (2454Kb) | Preview

Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)

Penelitian ini bertujuan mengevaluasi aktivitas antifungi ekstrak kulit tanaman hujan emas (Senna multijuga) terhadap jamur Ganoderma boninense secara in vitro dan in silico. Ekstraksi dilakukan melalui maserasi menggunakan alkohol 96%, dilanjutkan fraksinasi etil asetat kemudian pemurnian dengan kolom kromatografi menggunakan eluen 100% CHCl3, 3% MeOH/CHCl3, 20% MeOH/CHCl3 dan100% MeOH. Uji in vitro dilakukan dengan metode difusi senyawa aktif terhadap isolat murni G. boninense dalam media PDA. Hasil uji in vitro menunjukkan hasil kolom kromatograf fraksi 100% CHCl3 menghasilkan laju pertumbuhan terendah (0,39 cm/hari). Fraksi eluat 100% CHCl3 di kolom kromatografi untuk mendapat senyawa yang lebih murni (Fraksi 1-5) dan di lakukan uji in vitro terhadap G. boninense. Fraksi 1 menunjukkan daya hambat tertinggi dibandingkan fraksi lainnya dengan laju pertumbuhan G. boninense sebesar 0,52 cm/hari dan persentase daya hambat 37% sehingga diuji lebih lanjut pada berbagai konsentrasi (0; 0,25; 0,5; 0,75; 1; 1,25; dan 1,5 ppm) terhadap G. boninense. Kosentrasi 1,5 ppm fraksi 1 menghasilkan daya hambat tertinggi (54 %) dan laju pertumbuhan 0,40 cm/hari Uji in silico terhadap senyawa hasil LC-MS menggunakan molecular docking senyawa trans-1,1'-Dimethyl-5,5'-azotetrazole dari kulit hujan emas (Senna multijuga) menghambat ATP-dependent RNA helicase G. boninense dengan binding affinity -6,4 kcal/mol dengan ikatan hidrogen dengan situs aktif protein, yaitu Ser310A dan Thr360A. Berdasarkan hasil tersebut, ekstrak kulit S. multijuga memiliki potensi sebagai sumber antifungi alami terhadap G. boninense. Kata kunci: Senna multijuga, Ganoderma boninense, antifungi, in vitro, in silico, docking molekuler This study aims to evaluate the antifungal activity of bark extract from the golden shower tree (Senna multijuga) against Ganoderma boninense through in vitro and in silico approaches. Extraction was carried out via maceration using 96% ethanol, followed by ethyl acetate fractionation and purification using column chromatography with eluents of 100% chloroform (CHCl3), 3% methanol in chloroform, 20% methanol in chloroform, and 100% methanol. The in vitro assay was conducted using the diffusion method against pure isolates of G. boninense on PDA medium. Results showed that the 100% CHCl3 chromatographic fraction had the lowest fungal growth rate (0.39 cm/day). This fraction was further separated into sub-fractions (Fr.1 to Fr.5), which were then tested against G. boninense. Among them, Fraction 1 exhibited the highest inhibitory effect with a fungal growth rate of 0.52 cm/day and an inhibition percentage of 37%. Fraction 1 was further tested at various concentrations (0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 ppm), where the 1.5 ppm concentration resulted in the highest inhibition (54%) and a fungal growth rate of 0.40 cm/day. The in silico analysis using molecular docking revealed that the compound trans-1,1'-Dimethyl-5,5'-azotetrazole, identified from the LC-MS profile of S. multijuga bark, inhibited ATP-dependent RNA helicase of G. boninense with a binding affinity of -6.4 kcal/mol. The compound formed hydrogen bonds with the protein's active site residues, Ser310A and Thr360A. These findings suggest that S. multijuga bark extract has promising potential as a natural antifungal agent against G. boninense. Keywords: Senna multijuga, Ganoderma boninense, antifungal, in vitro, in silico, molecular docking

Jenis Karya Akhir: Tesis (Masters)
Subyek: 600 Teknologi (ilmu terapan) > 630 Pertanian dan teknologi yang berkaitan
Program Studi: FAKULTAS PERTANIAN (FP) & PASCASERJANA > Prodi S2 Magister Teknologi Industri Pertanian
Pengguna Deposit: UPT . Siswanti
Date Deposited: 04 Nov 2025 04:32
Terakhir diubah: 04 Nov 2025 04:32
URI: http://digilib.unila.ac.id/id/eprint/92666

Actions (login required)

Lihat Karya Akhir Lihat Karya Akhir
Digital Library is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.