UJI ANTAGONIS ISOLAT Bacillus thuringiensis SEBAGAI PENGENDALI JAMUR Colletotrichum sp. PENYEBAB PENYAKIT ANTRAKNOSA PADA BUAH CABAI MERAH (Capsicum annuum)

YASMINIA , FRISKA SAPUTRA (2025) UJI ANTAGONIS ISOLAT Bacillus thuringiensis SEBAGAI PENGENDALI JAMUR Colletotrichum sp. PENYEBAB PENYAKIT ANTRAKNOSA PADA BUAH CABAI MERAH (Capsicum annuum). FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM, UNIVERSITAS LAMPUNG.

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ABSTRAK Penurunan kuantitas dan kualitas cabai pascapanen disebabkan karena adanya infeksi jamur patogen Colletotrichum sp. Infeksi Colletotrichum sp. pada cabai dapat dikendalikan dengan agen hayati. Salah satu agen hayati yang dapat digunakan yaitu bakteri Bacillus thuringiensis. Tujuan dilakukannya penelitian ini untuk mengetahui kemampuan antagonis Bacillus thuringiensis dalam menghambat pertumbuhan Colletotrichum sp. serta untuk mengetahui efektivitas Bacillus thuringiensis dalam mengurangi keparahan penyakit akibat Colletotrichum sp. pada buah cabai merah. Penelitian ini dilakukan menggunakan Rancangan Acak Lengkap (RAL) dengan 7 isolat Bacillus thuringiensis yang diujikan secara in vitro. Persentase penghambatan paling besar di ujikan secara in vivo sebanyak 9 ulangan dengan parameter pengamatan persentase keparahan penyakit. Kontrol positif uji in vivo menggunakan buah cabai merah yang dilukai dan diberi suspensi konidia jamur Colletotrichum sp. Data persentase keparahan penyakit dianalisis menggunakan uji One-Way ANOVA dan dilanjutkan dengan uji beda nyata terkecil taraf 5%. Hasil uji in vitro didapati 3 isolat Bacillus thuringiensis memiliki persentase penghambatan yang besar yakni isolat kode Bt2, Bt3, dan Bt6 dengan nilai 76,76%, 60,96% dan 100%. Hasil uji in vivo menunjukkan adanya perbedaan signifikan persentase keparahan penyakit antara isolat kode Bt2 dan Bt3 dengan Bt6. Isolat kode Bt6 mampu menekan pertumbuhan jamur Colletotrichum sp. dengan melilit hifa jamur yang menandakan adanya interaksi kompetisi dan mikroparasitisme antara jamur dengan bakteri. Selain itu, isolat kode Bt6 efektif digunakan sebagai agen hayati dalam menghambat pertumbuhan Colletotrichum sp. Kata Kunci: Antraknosa, Bacillus thuringiensis, Cabai Merah, Colletotrichum sp., Uji Antagonis ABSTRACT The decrease in quantity and quality of post-harvest chili is caused by infection with the pathogenic fungus Colletotrichum sp. Colletotrichum sp. infection in chili can be controlled with biological agents. One of the biological agents that can be used is the bacterium Bacillus thuringiensis. The purpose of this study was to determine the ability of the antagonist Bacillus thuringiensis in inhibiting the growth of Colletotrichum sp. and to determine the effectiveness of Bacillus thuringiensis in reducing the severity of disease caused by Colletotrichum sp. in red chili fruit. This study was conducted using a Completely Randomized Design (CRD) with 7 isolates of Bacillus thuringiensis tested in vitro. The largest percentage of inhibition was tested in vivo with 9 replications with the observation parameter of the percentage of disease severity. The positive control of the in vivo test used injured red chili fruit and was given a suspension of Colletotrichum sp. conidia of the fungus. Data on the percentage of disease severity were analyzed using the One-Way ANOVA test and continued with the smallest significant difference test at a level of 5%. The results of the in vitro test found 3 isolates of Bacillus thuringiensis that had a large percentage of inhibition, namely isolates code Bt2, Bt3, and Bt6 with values of 76.76%, 60.96% and 100%. The results of the in vivo test showed a significant difference in the percentage of disease severity between isolates code Bt2 and Bt3 with Bt6. Isolate code Bt6 was able to suppress the growth of Colletotrichum sp. by wrapping around the fungal hyphae indicating the interaction of competition and microparasitism between fungi and bacteria. In addition, isolate code Bt6 is effective as a biological agent in inhibiting the growth of Colletotrichum sp. Keyword: Anthracnose, Bacillus thuringiensis, Red Chili, Colletotrichum sp., Antagonist test

Jenis Karya Akhir: Skripsi
Subyek: 500 ilmu pengetahuan alam dan matematika
500 ilmu pengetahuan alam dan matematika > 570 Biologi
Program Studi: FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM (FMIPA) > Prodi S1 Biologi
Pengguna Deposit: A.Md Cahya Anima Putra .
Date Deposited: 18 Nov 2025 03:30
Terakhir diubah: 18 Nov 2025 03:30
URI: http://digilib.unila.ac.id/id/eprint/93534

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