Tuti , Adawiyah (2025) PROFIL HEMATOLOGI DAN LIPID DROPLET HEPATOPANKREAS UDANG VANAME (Litopenaeus vannamei) PADA UJI LAPANG SUPLEMENTASI KOMBINASI Na-ALGINAT DAN VITAMIN C. PERTANIAN, UNIVERSITAS LAMPUNG .
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Abstract
Immunostimulation supplementation of Na-alginate and vitamin C has been proven to be effective in increasing the immunity of penaeus vannamei shrimp against pathogens on a laboratory scale. The purpose of this study was to deter- mine the effect of combined supplementation of Na-alginate Sargassum sp. and vitamin C in field tests on the hematological profile and hepatopancreas lipid droplets of penaeus vannamei shrimp (Litopenaeus vannamei). This study used two treatments, namely providing commercial feed without any additions (P1) and providing commercial feed with the addition of Na-alginate 100 ml/kg feed and vitamin C 3 g/kg feed (P2). Feeding was carried out at different levels. The study was conducted in a pond with an area of 3,500 m2 with a stocking density of 100 fish/m2 . Hemolymph sampling was carried out on DOC 35 and DOC 50 to obser- ve the number of total hemocytes (THC), total plasma protein (TPP), and hepato- pancreas lipid droplets and to check air quality. The research data by the T test, and the data quality was analyzed descriptively. The results of the study by adding Na-alginate supplementation of 100 ml/kg feed and vitamin C 3 g/kg feed in vaname shrimp cultivation were able to increase THC in DOC 50 to 5.99 x106 cells/ml, TPP values in DOC 35 to 655 mg/ml, and hepatopancreas lipid droplet values ranging from 71% -77%. The conclusion is that the application of a combination of Na-alginate and vitamin C in vaname shrimp is expected to be used as the body's immunity against shrimp diseases. Keyword : hematology, hepatopancreatic lipid droplets, Na-alginate, Pacific white shrimp, vitamin C Suplementasi imunostimulan Na-alginat dan vitamin C terbukti efektif mening- katkan imunitas udang vaname terhadap patogen pada skala laboratorium. Tujuan dari penelitian ini adalah untuk menentukan pengaruh suplementasi kombinasi Na-alginat Sargassum sp. dan vitamin C pada uji lapang terhadap profil hema- tologi dan lipid droplet hepatopankreas udang vaname (Litopenaeus vannamei). penelitian ini menggunakan dua perlakuan yaitu pemberian pakan komersil tanpa penambahan apapun (P1) dan Pemberian pakan komersil dengan penambahan Na- alginat 100 ml/kg pakan dan vitamin C 3 g/kg pakan (P2). Penelitian dilakukan di tambak dengan luas 3.500 m2 dengan padat tebar 100 ekor/m2 . Pengambilan hemolim dilakukan di DOC 35 dan DOC 50 untuk mengamati total haemocyte count (THC), total protein plasma (TPP), dan lipid droplet hepatopankreas serta pengecekan kualitas air. Data hasil penelitian yang diperoleh ditabulasi menggu- nakan Microsoft Excel kemudian dilanjut dengan uji T, dan data kualitas air di- analisis secara deskriptif. Hasil penelitian dengan menambahkan suplementasi Na- alginat 100 ml/kg pakan dan vitamin C 3 g/kg pakan pada budi daya udang vana- me mampu meningkatkan THC pada DOC 50 hingga 5,99 x106 sel/ml, nilai TPP pada DOC 35 hingga 655 mg/ml, dan nilai lipid droplet hepatopankreas berkisar 71%-77%. Kesimpulannya adalah pengaplikasian kombinasi Na-alginat dan vitamin C pada udang vaname diharapkan dapat digunakan sebagai imun tubuh terhadap penyakit udang. Kata Kunci : hematologi, lipid droplet hepatopankreas, Na-alginat, Udang vaname, vitamin C.
| Item Type: | Other |
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| Subjects: | ?? 630 ?? |
| Divisions: | ?? laut ?? |
| Depositing User: | . . Yulianti |
| Date Deposited: | 20 Nov 2025 03:16 |
| Last Modified: | 20 Nov 2025 03:16 |
| URI: | http://digilib.unila.ac.id/id/eprint/93675 |
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