Adila , - (2025) EKSPLORASI BAKTERI ENDOFIT TEBU (Saccharum officinarum L.) YANG BERPOTENSI SEBAGAI ENTOMOPATOGEN DAN PELARUT FOSFAT. FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG.
|
File PDF
ABSTRAK-1 - Adila Dizel.pdf Download (68Kb) | Preview |
|
![[img]](http://digilib.unila.ac.id/style/images/fileicons/text.png) |
File PDF
SKRIPSI FULL - Adila Dizel.pdf Restricted to Hanya staf Download (1537Kb) | Minta salinan |
|
|
File PDF
SKRIPSI TANPA BAB PEMBAHASAN - Adila Dizel.pdf Download (844Kb) | Preview |
Abstrak (Berisi Bastraknya saja, Judul dan Nama Tidak Boleh di Masukan)
Penelitian ini bertujuan untuk mengisolasi bakteri endofit dari tanaman tebu (Saccharum officinarum L.) yang berpotensi sebagai agen pengendali hayati hama dan pelarut fosfat. Skrining dilakukan dengan dua metode utama. Untuk menguji kemampuan bakteri sebagai entomopatogen, isolat bakteri (107 CFU/mL) disemprotkan pada larva Tenebrio molitor yang diletakkan dalam cawan petri (10 larva/cawan) dengan tiga ulangan. Larva diinkubasi selama 14 hari pada suhu ruang dengan pemberian pakan pur. Selanjutnya, untuk menguji kemampuan melarutkan fosfat, isolat bakteri diinokulasikan pada media Pikovskaya dengan empat ulangan. Sebanyak 21 isolat bakteri endofit tebu mampu menyebabkan mortalitas 100% terhadap larva uji dan sebanyak 98 isolat bakteri endofit tebu mampu melarutkan fosfat. Tiga isolat terpilih (G3D2.11 BKK, G5B2.160 KB, dan G7D2.176 BB) memiliki daya mortalitas 100% pada larva uji, tergolong bakteri Gram positif dan bersifat fermentatif, menunjukkan reaksi negatif pada uji soft rot dan hipersensitif serta bersifat hipovirulen yang artinya tidak bersifat patogen terhadap tanaman, serta tergolong sebagai bakteri pelarut fosfat tinggi. Hasil ini menunjukkan potensi besar isolat bakteri endofit tebu sebagai agen pengendalian hayati yang efektif serta berpotensi dalam meningkatkan ketersediaan fosfat bagi tanaman, yang mendukung keberlanjutan pertanian tebu. Kata kunci: bakteri endofit, entomopatogen, pelarut fosfat, pengendalian hayati, Tenebrio molitor This research aimed to isolate endophytic bacteria from sugarcane (Saccharum officinarum L.) with potential as biological control agents against pests and as phosphate solubilizers. Screening was conducted using two main methods. To test the bacteria's entomopathogenic potential, bacterial isolates (107 CFU/mL) were sprayed onto Tenebrio molitor larvae placed in petri dishes (10 larvae/dish) with three replications. The larvae were incubated for 14 days at room temperature and fed with standard feed. To assess phosphate solubilizing ability, bacterial isolates were inoculated onto Pikovskaya medium with four replications. A total of 21 endophytic bacterial isolates from sugarcane caused 100% mortality in the test larvae, and 98 isolates were capable of solubilizing phosphate. Three selected isolates (G3D2.11 BKK, G5B2.160 KB, and G7D2.176 BB) demonstrated 100% larval mortality, were identified as Gram-positive and fermentative, showed negative results in soft rot and hypersensitivity tests, and were classified as hypovirulent (non-pathogenic to plants). These isolates were also categorized as strong phosphate solubilizers. The results highlight the significant potential of endophytic bacterial isolates from sugarcane as effective biological control agents and in enhancing phosphate availability for plants, thereby supporting sustainable sugarcane agriculture. Keywords: endophytic bacteria, entomopathogen, phosphate solubilizer, biological control, Tenebrio molitor
| Jenis Karya Akhir: | Skripsi |
|---|---|
| Subyek: | 600 Teknologi (ilmu terapan) > 630 Pertanian dan teknologi yang berkaitan |
| Program Studi: | FAKULTAS PERTANIAN (FP) & PASCASERJANA > Prodi S1 Proteksi Tanaman |
| Pengguna Deposit: | UPT . Siswanti |
| Date Deposited: | 21 Nov 2025 02:32 |
| Terakhir diubah: | 21 Nov 2025 02:32 |
| URI: | http://digilib.unila.ac.id/id/eprint/93712 |
Actions (login required)
 |
Lihat Karya Akhir |
